Abstract: Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.

Abstract: A thermal mass flow sensor 10 enclosed airtightly in a sealed container 11 under an inert atmosphere for the purpose of suppressing disappearance of a coating layer on sensor wires 13a and 13b in association with use at a high temperature, further comprises an air release pipe 16 that is a pipe which brings an internal space and outside of the sealed container 11 in airtight communication with each other through an air release hole 16a that is a through-hole formed in an outer wall of the sealed container 11. An end of the air release pipe 16 on an opposite side to the air release hole 16a is sealed by plastic deformation to form a sealed part 16b. Thereby, after forming the sealed container 11 under a normal atmosphere, the internal space of the sealed container 11 can be closed airtightly. The sealed part 16b may be further sealed by welding.

Abstract: In a flow control valve which comprises a valve seat comprising an inner cylindrical part with a contact surface formed in its upper end part and an outer cylindrical part higher than the inner cylindrical member and a valve body that carries out an opening and closing action in a direction perpendicular to the contact surface of the valve seat, an inner cylindrical member and an outer cylindrical member, which are separate members, are airtightly fitted with each other to constitute the inner cylindrical part and the outer cylindrical part. As a result, by processing the contact surface of the inner cylindrical part to be smooth, the flow control valve can control flow rate of gas in a small flow rate range more accurately than before.

Abstract: Mass flow sensors, mass flow meters, and methods of making the same are disclosed. A flow sensor may include one sensor tube, a pair of sensor wires wound around the sensor tube, and a covering layer disposed in the surroundings of the sensor tube and the sensor wires. The covering layer includes a first covering layer disposed in contact with the surface of the sensor tube, a second covering layer disposed in contact with the surface of the sensor wires, a third covering layer disposed in a space surrounded by the first covering layer and the second covering layer, and a fourth covering layer disposed so as to cover the whole of the sensor wires wound around the sensor tube. In variations, the covering layer includes one or two organic materials chosen from polyamide-imide and polyimide, and the film thickness of the first covering layer is 10 micrometers or more.

Abstract: In a flow control valve which comprises a valve seat comprising an inner cylindrical part with a contact surface formed in its upper end part and an outer cylindrical part higher than the inner cylindrical member and a valve body that carries out an opening and closing action in a direction perpendicular to the contact surface of the valve seat, an inner cylindrical member and an outer cylindrical member, which are separate members, are airtightly fitted with each other to constitute the inner cylindrical part and the outer cylindrical part. As a result, by processing the contact surface of the inner cylindrical part to be smooth, the flow control valve can control flow rate of gas in a small flow rate range more accurately than before. It is preferable that arithmetic average roughness of the contact surface of the valve seat is 100 nanometers or less.

Abstract: A thermal mass flow sensor 10 enclosed airtightly in a sealed container 11 under an inert atmosphere for the purpose of suppressing disappearance of a coating layer on sensor wires 13a and 13b in association with use at a high temperature, further comprises an air release pipe 16 that is a pipe which brings an internal space and outside of the sealed container 11 in airtight communication with each other through an air release hole 16a that is a through-hole formed in an outer wall of the sealed container 11. An end of the air release pipe 16 on an opposite side to the air release hole 16a is sealed by plastic deformation to form a sealed part 16b. Thereby, after forming the sealed container 11 under a normal atmosphere, the internal space of the sealed container 11 can be closed airtightly. The sealed part 16b may be further sealed by welding.

Abstract: A game machine comprises a physical lottery mechanism including a plurality of lottery portions, wherein an image display device having a transmissive screen through which each lottery portion can be seen is arranged on each of the front and back of the lottery mechanism; games different from each other are simultaneously performed so as to make a difference between a lottery portion to be used in a game for one side of the lottery mechanism and a lottery portion to be used in a game for the other side of the lottery mechanism, and display of the image display device is controlled so that a lottery state of a lottery portion being used in a game is seen through the transmissive region of the screen, while a lottery state of a lottery portion not being used in the game is less visible or invisible in the game.

Abstract: A gaming system provides a game to one or more players and includes a ball lottery machine. The ball lottery machine includes a frame, a lottery ball field, an image projection unit, and a control unit. The lottery ball field has a rotating field. The rotating field includes a plurality of ball pockets spaced on the rotating field. The ball pockets are configured to receive a lottery ball. The rotating field is rotatably coupled to the frame. The image projection unit is coupled to the frame and is configured to project a rotating image into an upper surface of the rotating field. The control unit is configured to establish a rate of rotation associated with the rotating field and to responsively control a rate of rotation of the rotating image using the image projection unit.

Abstract: A gaming system provides a game to one or more players and includes a ball lottery machine. The ball lottery machine includes a frame, a lottery ball field, an image projection unit, and a control unit. The lottery ball field has a rotating field. The rotating field includes a plurality of ball pockets spaced on the rotating field. The ball pockets are configured to receive a lottery ball. The rotating field is rotatably coupled to the frame. The image projection unit is coupled to the frame and is configured to project a rotating image into an upper surface of the rotating field. The control unit is configured to establish a rate of rotation associated with the rotating field and to responsively control a rate of rotation of the rotating image using the image projection unit.

Abstract: A first thermal shield constituted by a material with a thermal conductivity of 20 W/(m·K) or less and a second thermal shield constituted by a space are disposed between a passage and a laminated piezoelectric actuator. Preferably, a third thermal shield with a thermal conductivity of 20 W/(m·K) or less is further disposed. Thereby, a flow control valve which can effectively intercept a transmission of heat using a simpler and compacter structure than a flow control valve according to a conventional technology so that the temperature of a laminated piezoelectric actuator does not exceed its heat-resistant temperature even when it is used for high-temperature process gas is provided.

Abstract: A game system includes a selection device and a game control device. The selection device may be configured to select, for selecting one or more base games, at least one of a) the number of one or more primary games; and b) a type of the one or more base games, using at least a predefined information. A game control device may be configured to start or advance a secondary game, which is subordinate to the one or more primary games, based at least in part on a game state of the one or more base games selected by the selection device.

Abstract: A game machine comprises a physical lottery mechanism including a plurality of lottery portions, wherein an image display device having a transmissive screen through which each lottery portion can be seen is arranged on each of the front and back of the lottery mechanism; games different from each other are simultaneously performed so as to make a difference between a lottery portion to be used in a game for one side of the lottery mechanism and a lottery portion to be used in a game for the other side of the lottery mechanism, and display of the image display device is controlled so that a lottery state of a lottery portion being used in a game is seen through the transmissive region of the screen, while a lottery state of a lottery portion not being used in the game is less visible or invisible in the game.

Abstract: A first thermal shield constituted by a material with a thermal conductivity of 20 W/(m·K) or less and a second thermal shield constituted by a space are disposed between a passage and a laminated piezoelectric actuator. Preferably, a third thermal shield with a thermal conductivity of 20 W/(m·K) or less is further disposed. Thereby, a flow control valve which can effectively intercept a transmission of heat using a simpler and compacter structure than a flow control valve according to a conventional technology so that the temperature of a laminated piezoelectric actuator does not exceed its heat-resistant temperature even when it is used for high-temperature process gas is provided.

Abstract: Among covering layers constituting a covering layer 4 of a flow sensor 1, a second covering layer 42 disposed in contact with the surface of a sensor wire 3 has a configuration in which an inorganic material finely dispersed in a base material consisting of an organic material and the film thickness of a first covering layer 41 disposed in contact with the surface of a sensor tube 2 is 10 micrometers or more. An interval d between the adjacent second covering layers 42 is preferably 10 micrometers or less. Thereby, a flow sensor which can be used longer than a flow sensor according to a conventional technology even when it is used at a high temperature in order to prevent the condensation of condensable gas is provided. Furthermore, a reduction of the electrical resistance of the sensor wire associated with the use in an elevated temperature is suppressed by an annealing treatment under an inert gas atmosphere and a predetermined condition, after a formation of a mass flow sensor.

Abstract: In the game machine which is provided with a lottery device which executes a lottery and causes a predetermined privilege when a predetermined condition is satisfied and a control unit, the control unit receives applications for participation of a plurality of players in a lottery by the lottery device on a condition of paying chips, executes a lottery by the lottery device when a total of the number of chips paid by the plurality of players whose applications for participation have been received reaches a predetermined specified number, and distributes a privilege decided by the lottery device according to a ratio of the number of chips paid by each player whose application for participation has been received.

Abstract: In a game system which is provided with a monitor which displays a screen according to progress of a game, a touch panel which is operated by a player according to the progress of a game, a storage device which stores operation history data of the player who operates the touch panel, and a control unit, the control unit determines an attribute of the player based on the operation history data and controls setting of a game based on the determined attribute.

Abstract: In a store information presentation system which is connected to perform communication with a plurality of game machines installed in stores, a server and a user terminal which transmits position information to the server, each of the plurality of game machines transmits installation information including a game machine code used to individually identify the game machine and a store code used to individually identify the store to the server, the server manages the game machine code and the store code, specifies the store closest the position represented by the position information received from the user terminal with reference to the game machine list and the store list and transmits store information of specified store to the user terminal, and the user terminal presents a user with the store information received from the server.

Abstract: In the game machine which is provided with a lottery device which executes a lottery and causes a predetermined privilege when a predetermined condition is satisfied and a control unit, the control unit receives applications for participation of a plurality of players in a lottery by the lottery device on a condition of paying chips, executes a lottery by the lottery device when a total of the number of chips paid by the plurality of players whose applications for participation have been received reaches a predetermined specified number, and distributes a privilege decided by the lottery device according to a ratio of the number of chips paid by each player whose application for participation has been received.

Abstract: In a game machine which is provided with an input interface having a configuration in which a game field on which a game progresses according to movement of medals, a monitor, and a touch panel superimposed on the monitor are combined, and a control unit, the control unit causes a digital medal associated with the medal to be displayed on the monitor, causes the digital medal to be associated with the player's touch operation, causes the digital medal to operate according to a predetermined operation condition, applies an operation associated with an operation of the digital medal to the medal, and selects the operation condition according to a predetermined selection condition from among a plurality of candidates of the operation condition.

Abstract: In a game system which is provided with a monitor which displays a screen according to progress of a game, a touch panel which is operated by a player according to the progress of a game, a storage device which stores operation history data of the player who operates the touch panel, and a control unit, the control unit determines an attribute of the player based on the operation history data and controls setting of a game based on the determined attribute.