Abstract: By using a biologically active substance-immobilized device, which comprises a base particle comprising a core particle and an organic compound having two or more hydrophilic groups and immobilized on the core particle by a chemical bond and a biologically active substance bonded to the base particle via the organic compound, a substance specifically bonding to the biologically active substance is detected or measured.

Abstract: A solution of a nucleic acid is spotted on a metal carrier consisting of a metal selected from Groups I, II, III, IV, V, VI, and VII of second to seventh periods and transition elements in a periodic table, or of an alloy containing the metal, and the solution is dried. Then, the nucleic acid is immobilized on the carrier spotted with the solution by irradiating the carrier with an ultraviolet ray containing a component having a wavelength of 280 nm, of which irradiation dose is 100 mJ/cm2 or more.

Abstract: A nucleic acid is immobilized on a carrier by spotting a solution of the nucleic acid on the carrier comprising a synthetic resin such as polycarbonate, polymethyl methacrylate, acrylonitrile/butadiene/styrene copolymer, polyethylene, polyethylene terephthalate, polyphenol, polystyrene, polyacrylonitrile, polyvinyl chloride, or aramide, drying the solution, and irradiating the carrier with an ultraviolet ray containing a component having a wavelength of 280 nm, preferably for 100 mJ/cm2 or more.