Abstract: A detection method of a target molecule in a specimen includes a (target molecule)-(labeled binding molecule)-(capturing molecule) complex forming step of reacting the target molecule in the specimen, a capturing molecule that binds to the target molecule, and a labeled binding molecule that binds to the target molecule and which is labeled with an enzyme that catalyzes a reaction to produce ATP, to form a complex formed of the target molecule, the capturing molecule, and the labeled binding molecule, a step of removing the labeled binding molecules which did not bind to the target molecule, an ATP production step of producing ATP by reacting the (target molecule)-(labeled binding molecule)-(capturing molecule) complex with a substrate of the enzyme that catalyzes a reaction to produce ATP, an ATP amplification step of amplifying the produced ATP, and an ATP detection step of detecting the amplified ATP.

Abstract: To provide an Al—Mg—Si-based aluminum alloy sheet excellent in formability with excellent breaking elongation and work hardenability. An Al—Mg—Si-based aluminum alloy sheet excellent in formability contains Mg: 0.3 mass % or more and 0.45 mass % or less and Si: 0.6 mass % or more and 1.75 mass % or less with the balance being Al and inevitable impurities, in which, when content of the Mg is expressed [Mg] in terms of mass % and content of the Si is expressed [Si] in terms of mass %, [Si]/[Mg] is more than 2.5, a height of a first exothermic peak appearing in a temperature range of 210° C. or above and below 260° C. in a differential scanning thermal analysis curve is 20 ?W/mg or more, and a height of a second exothermic peak appearing in a temperature range of 260° C. or above and 370° C. or below in a differential scanning thermal analysis curve is 18 ?W/mg or more.

Abstract: A selection method of an iPS cell includes: at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell; judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number; and when the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process.

Abstract: A cell specifying method includes, for a first cell population including cells into which a nucleic acid encoding plural kinds of reprogramming factors necessary for reprogramming of somatic cells, and a nucleic acid encoding a luminescent reporter protein configured to be co-expressed with a kind of the plural kinds of reprogramming factors are introduced, acquiring a first luminescence image concerning luminescence caused by expression of the luminescent reporter protein, dividing the first cell population into second cell populations, for the second cell populations, acquiring a second luminescence image concerning luminescence caused by expression of the luminescent reporter protein, selecting objective cells from the first cell population based on the first luminescence image, and specifying the objective cells in the second cell populations based on the first luminescence image and the second luminescence image.

Abstract: The disclosed technology provides a method for analyzing a somatic cell reprogramming, the method including acquiring luminescence images associated with luminescences attributed to expression of luminescent reporter proteins during a period of culturing cells transfected with a nucleic acid encoding plural types of transcription factors required for the somatic cell reprogramming and a nucleic acid encoding the luminescent reporter proteins configured to be co-expressed with at least one of the plural types of transcription factors in S12, quantifying luminescence intensities of the luminescences based on the luminescence images in S13, and evaluating expression states of the transcription factors to be co-expressed with the luminescent reporter proteins based on the luminescence intensities in S14, in which the expression states are indicators for evaluating whether a pluripotency has been acquired in the cell reprogramming process.

Abstract: A method for assessing irritancy to a biological sample of the present invention is a method for assessing irritancy of a chemical to a three-dimensional biological sample having a plurality of layers in the depth direction and includes: image acquisition step of acquiring, at different depths of the biological sample, fluorescence images of a nuclei of cells in at least one group of a living cell group and a dead cell group in the biological sample exposed to the chemical, the nuclei of cells in at least one group being labeled with a fluorescent marker; and assessment step of calculating the ratio of the number of cells in the at least one cell group existing in the depth direction on the basis of the plurality of fluorescence images.

Abstract: The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.

Abstract: The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

Abstract: The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

Abstract: According to one embodiment, the present invention relates to luciferase derived from Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.

Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.

Abstract: The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

Abstract: According to one embodiment, the present invention relates to luciferase derived from Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.

Abstract: Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.

Abstract: Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.

Abstract: A novel apparatus and a novel method of imaging a luminescence phenomenon of a sample of biological origin using an optical image formation device are provided. In the inventive method and apparatus, an illumination image acquired by illuminating the sample and a luminescence image acquired with the light emitted by a luminescence phenomenon of a cell in the sample without illuminating it are superposed to generate a superposition image. In the superposition image, an analysis region is determined. Thereby, even when the light detected in a luminescence observation is feeble, a luminescent cell and/or a luminescent site in a cell in a sample can be specified.

Abstract: Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.