Abstract: A method for detecting a biological species of interest (SOI) potentially present in an analysis sample, the biological species of interest having a known or partially known genome, the analysis sample comprising a mixture of various biological species, the method comprising (a) extracting nucleic acids from the analysis sample; (b) sequencing nucleotide sequences extracted, (c) on the basis of the sequencing result, (i) assigning the sequences resulting from the sequencing, based on a reference database of sequences; (i) assigning sequences; (ii) determining a quantity (RSOI, RNSOI) of sequences assigned to the biological species of interest; and, prior to the sequencing, adding a calibrator, the calibrator being a biological species added in a known concentration, to the analysis sample, the calibrator having a known genome, and on the basis of the sequencing result, determining a quantity (RCAL) of sequences assigned to the calibrator; (d) on the basis of the quantities of sequences estimated in the det

Abstract: A method for detecting a biological species of interest (SOI) present in an analysis sample, the biological species of interest having a known genome, the analysis sample comprising a mixture of various biological species, the method comprising (a) extracting nucleotide sequences from the analysis sample; (b) sequencing the nucleotide sequences extracted in the extracting; (c) on the basis of the sequencing, obtaining normalized sequences assigned to the biological species of interest and to the control species; and (d) comparing the normalized sequences respectively assigned to the biological species of interest and to the control species to detection thresholds respectively associated with the biological species of interest and with the control species.