Abstract: There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods suffer from a lack of sensitivity, low throughput, and are labor intensive. The claimed methods provide fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.

Abstract: A hybridoma cell line producing high levels of human sequence antibody. Also described is a hybridoma cell line producing human sequence antibodies that is adapted for growth in serum-free media or animal-derived-components-free media. A hybridoma cell line producing anti-CTLA4 antibodies is most preferred.