Patents by Inventor Sa{hacek over (s)}a Stojkovic

Sa{hacek over (s)}a Stojkovic has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for controlling the main complex N-glycan structures and the acidic variants and variability in bioprocesses producing recombinant proteins
    Patent number: 9447188
    Abstract: The present invention relates to a method of controlling quality and quantity of posttranslational modification of a recombinantly produced polypeptide/protein (glycoprotein), wherein the posttranslational modification affects the glycosylation profile and/or the acidic variants profile, as manifested in CEX profiles, wherein the polypeptide/protein (glycoprotein) production is in eukaryotic host cells, the method comprising the following steps: a) cultivating the eukaryotic cells in a suitable medium under conditions which allow the expression of the polypeptide/protein, wherein the content of the dissolved CO2 (pCO2) in the medium is at a first value during the initial growth phase of the eukaryotic cells, allowing the eukaryotic cells to grow, and b) increasing or decreasing the content of the dissolved CO2 (pCO2) in the medium during the production phase of the eukaryotic cells to a second value.
    Type: Grant
    Filed: April 12, 2012
    Date of Patent: September 20, 2016
    Assignee: LEK PHARMACEUTICALS D.D.
    Inventors: Matja{hacek over (z)} Koncilja, Vatroslav Spudic, Sa{hacek over (s)}a Stojkovic, Matja{hacek over (z)} Tisu