Abstract: In accordance with various embodiments of the disclosed subject matter, a system, device and platform for culturing and maintaining tissue representative cellular models combined with active fluidics mimicking body circulation. In combination, multiple chamber devices enable modeling of multi-organ communication, representative of in vivo conditions.

Abstract: A nasal simulator includes a three-dimensional (3D) printed nasal cavity within based on diagnostic imagery of a human nasal cavity. The nasal simulator comprising a fan system positioned to mimic air flow through the human nasal cavity. A first probe access bore is formed through the 3D printed nasal cavity to a first location having a first internal contour. An anemometer insert having an outer diameter sized to be slidingly received in and to pneumatically seal the first probe access bore, the anemometer insert having a distal contour that aligns with the first internal contour of the 3D printed nasal cavity, the anemometer insert having a longitudinal bore that is sized to receive a probe of an anemometer to detect characteristics of the air flow through the 3D cavity.

Abstract: A nasal simulator includes a three-dimensional (3D) printed nasal cavity within based on diagnostic imagery of a human nasal cavity. The nasal simulator comprising a fan system positioned to mimic air flow through the human nasal cavity. A first probe access bore is formed through the 3D printed nasal cavity to a first location having a first internal contour. An anemometer insert having an outer diameter sized to be slidingly received in and to pneumatically seal the first probe access bore, the anemometer insert having a distal contour that aligns with the first internal contour of the 3D printed nasal cavity, the anemometer insert having a longitudinal bore that is sized to receive a probe of an anemometer to detect characteristics of the air flow through the 3D cavity.

Abstract: The anatomical model of a nasal cavity, such as a human nasal cavity, for in-vitro inhalation studies such as toxicological screening, intranasal drug delivery studies, and neurophysiological studies. The model includes a model body including separable upper and lower model portions together defining the nasal cavity and including fluidic channels therein that define an olfactory region of the upper model portion, and a nasal passage defined in the lower model portion. A biocompatible porous membrane is positioned between the upper and lower model portions, and the biocompatible membrane is configured for culturing olfactory epithelium cells thereon. An artificial mucous layer coats a surface of the nasal cavity and is configured to collect particles passing through the nasal cavity.

Abstract: 11-mercaptoundecyl trimethylammonium bromide gold nanorod (MTAB-GNR) coated with tannic acid (TA), useful for biological cellular imaging. A method of synthesizing tannic acid-coated 11-mercaptoundecyl trimethylammonium bromide gold nanorods (MTAB-TA GNRs) comprising presenting 11-mercaptoundecyl trimethylammonium bromide gold nanorods (MTAB-GNRs) in solution; functionalizing the MTAB-GNRs with tannic acid (TA) added to the MTAB-GNRs, the tannic acid configured to form a coating around the MTAB-GNRs forming MTAB-TA GNRs. The functionalizing step may further comprise adding a sodium chloride solution to the MTAB-TA GNR solution, and/or adding a MOPS buffer to the solution of tannic acid with MTAB-GNRs, and the tannic acid MTAB-GNR solution may be vortexed after each step.

Abstract: 11-mercaptoundecyl trimethylammonium bromide gold nanorod (MTAB-GNR) coated with tannic acid (TA), useful for biological cellular imaging. A method of synthesizing tannic acid-coated 11-mercaptoundecyl trimethylammonium bromide gold nanorods (MTAB-TA GNRs) comprising presenting 11-mercaptoundecyl trimethylammonium bromide gold nanorods (MTAB-GNRs) in solution; functionalizing the MTAB-GNRs with tannic acid (TA) added to the MTAB-GNRs, the tannic acid configured to form a coating around the MTAB-GNRs forming MTAB-TA GNRs. The functionalizing step may further comprise adding a sodium chloride solution to the MTAB-TA GNR solution, and/or adding a MOPS buffer to the solution of tannic acid with MTAB-GNRs, and the tannic acid MTAB-GNR solution may be vortexed after each step.

Abstract: A single- or multi-well chamber assembly comprising nanoparticle exposure chambers with a plurality of wells designed to hold porous membrane inserts, with cell culture media being maintained on the basal (lower) surface to generate the air-liquid interface. The wells may accommodate a variety of sizes of cell culture inserts and may be arranged radially. The chamber assembly comprises an integrated heating-humidifying mechanism so that the internal chamber environment may be heated to 37° C. and maintained at humid conditions to allow for maintenance of cell cultures. The presently disclosed apparatus may be used to investigate the toxicity of airborne nanoparticles to cells or tissue models grown at the air-liquid interface. Aerosolized nanomaterials are drawn into a multi-well chamber assembly and deposited via electrostatic deposition onto cells grown at the air-liquid interface.