Abstract: A filter cigarette includes a filter, a filter wrapper material enwrapping the filter, a tobacco rod, a tobacco rod wrapper material enwrapping the tobacco rod, and a mouthpiece wrapper material. The mouthpiece wrapper material enwraps at least a subregion of the filter wrapper material and has an upper surface which forms at least part of the surface of the filter cigarette. The filter cigarette has at least one fluorescent or phosphorescent substance in the region of the mouthpiece wrapper material below the upper surface of the mouthpiece wrapper material. The mouthpiece wrapper material has an opening in at least one region of the fluorescent or phosphorescent substance or is embodied as a translucent or transparent film, so that this at least one region is visible from the outside of the filter cigarette.

Abstract: A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.

Abstract: A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.