Abstract: Kit, probe mixture and probes for the detection of a chromosome aberration. Genetic probe, obtained by a method comprising the following steps: (a) Examine genomic segments for sequence regions with non-repetitive nucleic acid sequences and select one or more nucleic acid sequences; (b) Design and synthesize primer pairs for a polymerase chain reaction on the non-repetitive nucleic acid sequences, where the primers each have an oligonucleotide sequence which is complementary to the strand or complementary strand of the non-repetitive nucleic acid sequence, and a non-complementary universal linker sequence; (c) Carry out a first PCR and obtain a first mixture (pool A) of nucleic acid fragments; (d) Carry out a multiplex PCR on the mixture (pool A) with the use of primers which hybridize onto the linkers, and obtain a mixture (pool B) with amplified, non-repetitive nucleic acid fragments which are suitable for chromogenic or fluorescence in-situ hybridization of chromosomes (FISH/CISH/ISH).