Patents by Inventor Sachio Miyairi

Sachio Miyairi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme
    Patent number: 5635378
    Abstract: There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: June 3, 1997
    Assignee: Director-General of Agency of Industrial Science and Technology
    Inventors: Ikuo Matsui, Kazuhiko Ishikawa, Sachio Miyairi, Koichi Honda
  • Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme
    Patent number: 5631149
    Abstract: There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: May 20, 1997
    Assignee: Director-General Of Agency of Industrial Science and Technology
    Inventors: Ikuo Matsui, Kazuhiko Ishikawa, Sachio Miyairi, Koichi Honda
  • Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme
    Patent number: 5538882
    Abstract: There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
    Type: Grant
    Filed: March 2, 1994
    Date of Patent: July 23, 1996
    Assignee: Director-General of Agency of Industrial Science and Techology
    Inventors: Ikuo Matsui, Kazuhiko Ishikawa, Sachio Miyairi, Koichi Honda
  • Enzyme immobilization with azido compounds
    Patent number: 4160698
    Abstract: A given enzyme is immobilized by a method comprising the steps of mixing the enzyme with an azido compound and a high molecular compound as a carrier, forming the resultant mixture in a desired shape, drying the formed mixture and exposing the formed and dried mixture to light for thereby causing the enzyme to be bound to the azido group of the azido compound and to be bound to the high molecular compound.
    Type: Grant
    Filed: September 8, 1977
    Date of Patent: July 10, 1979
    Assignee: Agency of Industrial Science & Technology, Ministry of International Trade & Industry
    Inventors: Sachio Miyairi, Hideaki Tanaka, Akira Yabe, Koichi Honda