Abstract: It is intended to provide a method for determining a tumor. The method for determining a tumor comprises: (1) treating genomic DNA prepared from a subject tissue or cell with bisulfite (the subject tissue or cell is derived from a patient who is affected by a tumor and is determined as (i) having MSI-H of the tumor in MSI examination and/or no or reduced expression of MLH1 in the tumor in immunohistochemical examination, and (ii) having no mutation in MLH1 in genetic examination); 2) amplifying, by PCR, DNA comprising a portion or the whole of MLH1 promoter region from the bisulfite-treated DNA; 3) subjecting the PCR amplification product to ion exchange chromatography to obtain a detection signal; 4) determining whether or not the peak of the detection signal is a peak indicating highly methylated DNA; and 5) determining the tumor as a tumor derived from a patient without Lynch syndrome when the peak is determined as a peak indicating highly methylated DNA.

Abstract: It is intended to provide a method for determining a tumor. The method for determining a tumor comprises: (1) treating genomic DNA prepared from a subject tissue or cell with bisulfite (the subject tissue or cell is derived from a patient who is affected by a tumor and is determined as (i) having MSI-H of the tumor in MSI examination and/or no or reduced expression of MLH1 in the tumor in immunohistochemical examination, and (ii) having no mutation in MLH1 in genetic examination); 2) amplifying, by PCR, DNA comprising a portion or the whole of MLH1 promoter region from the bisulfite-treated DNA; 3) subjecting the PCR amplification product to ion exchange chromatography to obtain a detection signal; 4) determining whether or not the peak of the detection signal is a peak indicating highly methylated DNA; and 5) determining the tumor as a tumor derived from a patient without Lynch syndrome when the peak is determined as a peak indicating highly methylated DNA.

Abstract: Provided is a detection method employing a detection instrument having a plurality of wells at a surface thereof, the method including depositing a detection object, preferably in coexistence with a specific holding substance, onto the inner walls of the wells of the detection instrument; bringing a reaction solution into contact with the inner walls of the wells of the detection instrument on which the detection object has been deposited, the reaction solution containing a substance reactive with the detection object; and detecting a signal generated through this contact, to thereby evaluate the detection object. In this method, a liquid phase reaction can be efficiently performed in the detection instrument for carrying out a biological reaction (e.g., a microarray) so that samples contained in the wells are not mixed with one another, and that air bubbles are prevented from being generated in the wells.

Abstract: There are provided a) a detection board having numerous wells on a surface thereof, b) a detection set including the detection board and an element serving as well sealing means, and c) a method of using the detection set b), which includes injecting, into each of the wells of the detection board, an element for detecting a liquid phase reaction, sealing the element into the well with the well sealing means, and detecting the liquid phase reaction in the well. Thus, there is provided means for efficiently performing a liquid phase reaction on the board in a manner similar to that of an existing microarray technique.

Abstract: A tumor suppressor gene (TSLL1 gene) having a nucleotide sequence encoding an amino acid sequence of SEQ ID NO: 1. The gene is useful in the prevention and treatment of cancers. Proteins, vectors, transformants and antibodies related to the gene are also disclosed.

Abstract: A tumor suppressor gene (TSLL2 gene) having a nucleotide sequence encoding an amino acid sequence of SEQ ID NO: 1. The gene is useful in the prevention and treatment of cancers. Proteins, vectors, transformants, and antibodies related to the gene are also disclosed.

Abstract: Process for preparing fluorine-containing copolymers by using vinyl ether monomer compositions comprising vinyl ethers having a carboxyl acid salt in an amount of 1 to 50% by mole of vinyl ethers having a free carboxyl group. According to the process, the amount of halogen generated can be reduced.