Abstract: The present invention provides an genetically engineered cDNA of the rat bcl-x gene, which has at least one substitution selected from the substitutions that change residues 22 Tyr to Phe, residues 26 Gln to Asn and residues 165 Arg to Lys, in the coding region of rat bcl-x cDNA of SEQ ID NO: 1, a recombinant vector containing the engineered cDNA, a cell into which the recombinant vector was introduced, and an improved protein of Bcl-xL. The improved protein of Bcl-xL is useful as an ingredient for remedies for various diseases accompanied with cell death since it effectively inhibit cell death such as apoptosis.

Abstract: The present invention provides a protein having a potent cell-death inducing activity that is, a fused protein is in which a modified Bax protein fused with GFP at the N-terminus and is further fused with a homing signal peptide having a homing activity specific for endothelial cells in tumor angiogenesis; a gene coding the fused protein; and a cancer cell growth inhibitor containing the fused protein. Namely, a fused gene containing a cell death-inducing gene acting specially on cancer tissue, which is a fused gene having a gene encoding a homing signal peptide sequence specific for endothelial cells undergoing angiogenesis, a gene encoding a green fluorescent protein (GFP) and a gene encoding ?NBax protein, which is human Bax with a deletion of the N-terminal sequence containing the BH3 domain in this order, and a fusion protein encoded by the fused gene.

Abstract: The present invention provides an genetically engineered cDNA of the rat bcl-x gene, which has at least one substitution selected from the substitutions that change residues 22 Tyr to Phe, residues 26 Gln to Asn and residues 165 Arg to Lys, in the coding region of rat bcl-x cDNA of SEQ ID NO: 1, a recombinant vector containing the engineered cDNA, a cell into which the recombinant vector was introduced, and an improved protein of Bcl-xL. The improved protein of Bcl-xL is useful as an ingredient for remedies for various diseases accompanied with cell death since it effectively inhibit cell death such as apoptosis.

Abstract: The present invention provides an genetically engineered cDNA of the rat bcl-x gene, which has at least one substitution selected from the substitutions that change residues 22 Tyr to Phe, residues 26 Gln to Asn and residues 165 Arg to Lys, in the coding region of rat bcl-x cDNA of SEQ ID NO: 1, a recombinant vector containing the engineered cDNA, a cell into which the recombinant vector was introduced, and an improved protein of Bcl-xL. The improved protein of Bcl-xL is useful as an ingredient for remedies for various diseases accompanied with cell death since it effectively inhibit cell death such as apoptosis.

Abstract: The present invention provides a protein having a potent cell-death inducing activity that is, a fused protein is in which a modified Bax protein fused with GFP at the N-terminus and is further fused with a homing signal peptide having a homing activity specific for endothelial cells in tumor angiogenesis; a gene coding the fused protein; and a cancer cell growth inhibitor containing the fused protein. Namely, a fused gene containing a cell death-inducing gene acting specially on cancer tissue, which is a fused gene having a gene encoding a homing signal peptide sequence specific for endothelial cells undergoing angiogenesis, a gene encoding a green fluorescent protein (GFP) and a gene encoding ?NBax protein, which is human Bax with a deletion of the N-terminal sequence containing the BH3 domain in this order, and a fusion protein encoded by the fused gene.