Abstract: Disclosed herein is a method of treating a subject for a disease, disorder, or condition that involves administering to the subject a composition comprising a secretome derived from electrically stimulated cells. Also disclosed herein is an enhanced secretome produced by a process comprising collecting cells from a subject, culturing the cells in a serum free medium for at least 1 day while stimulating the cells with electric pulses, and collecting the secretome produced by the electrically-stimulated cells. Also disclosed herein is a pharmaceutical composition comprising an enhanced secretome derived from electrically stimulated cells contained within a biocompatible hydrogel in an amount effective to treat a disease or disorder.

Abstract: Tissue-engineered nerve scaffolds, articles for up-selecting desired cell proliferation and down-selecting undesired cell proliferation, and methods of manufacturing the same are provided. The tissue-engineered nerve scaffold includes a hydrogel having a surface. The surface has a topography including a micropattern defined by a plurality of spaced features attached to or projected into the hydrogel. The micropattern facilitates attachment and alignment of neural cells and reduces attachment and alignment of cells associated with scar-tissue formation and encapsulation.