Abstract: Provided herein, in some embodiments, are engineered nucleic acid targeting vectors that include a sequence of interest flanked by homology arms, each homology arm comprising a sequence homologous to a sequence in a safe harbor site in the human genome in any one of the following loci: 1q31, 3p24, 7q35, and Xq21. Also provided herein are methods of using and compositions the comprising engineered nucleic acid targeting vectors.

Abstract: Aspects of the disclosure provide techniques for retransmitting transaction layer packets (TLPs) for which a negative acknowledgment (NACK) is received without retransmitting previously transmitted TLPs that are correctly received, yet out-of-sequence, by a receiver. A receiver (e.g., a receiving link partner) can provide a transmitter (e.g., a transmitting link partner) with a NACK that includes a starting sequence number (SSN) and an ending sequence number (ESN), which can notify the transmitter about the packets for retransmission and/or packets that can be purged from a transmit buffer of the transmitter.

Abstract: Non-acidic sulfur compounds (e.g., thiols) can be selectively extracted from a hydrocarbon stream containing them (e.g., crude oil) by contacting the hydrocarbon stream with a carbonate solvent that contains at least one organic carbonate (e.g., propylene carbonate) in an amount effective to selectively absorb and extract the non-acidic sulfur compounds therefrom. The carbonate solvent may optionally also comprise an additional solvent (e.g., caprolactam), a soluble metal salt (e.g., a metal salt where the metal is iron), and/or a mercaptan scavenger that is a heterocyclic amine (e.g., oxazolidine).

Abstract: It is a continuing goal to reduce the amount of sludge produced in a biorefinery while also improving oil quality and water quality. In one aspect, an exemplary method of minimizing sludge from biorefineries includes the steps of feeding a source feedstock into a degumming centrifuge, where the source feedstock is separated into wet oil and a primary sludge stream, and conveying a portion of the primary sludge stream to a three-phase centrifuge. At the three-phase centrifuge, the portion of the primary sludge stream is separated into recovered centrifuge oil, which is retrieved; centrifuged water, which is conveyed to a wastewater treatment plant; and centrifuged sludge. The remainder of the primary sludge stream is conveyed to a gravity separator, where the remainder of the primary sludge stream is separated into recovered oil and residual sludge. The residual oil is retrieved from the gravity separator.

Abstract: A device includes a memory configured to store data corresponding to a media stream including video recorded at a first frame rate and multiple audio segments includes the multiple audio segments include one or more first audio segments indicated as having a first playback type and one or more second audio segments indicated as having a second playback type includes the device also includes one or more processors coupled to the memory and operably configured to initiate playback of the media stream includes the playback of the media stream includes playback of the video at a second frame rate that is different from the first frame rate, playback of the one or more first audio segments at a first playback speed corresponding to the first frame rate, and playback of the one or more second audio segments at a second playback speed corresponding to the second frame rate.

Abstract: Catalyst fines can be removed from heavy oils, such as marine fuel oils, by introducing an additive in an effective amount to at least partially remove the catalyst fines, where the additive is an oxyalkylated acid-catalyzed alkylphenol formaldehyde resin and/or a Mannich condensate base resin copolymer.

Abstract: According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

Abstract: Catalyst fines can be removed from heavy oils, such as marine fuel oils, by introducing an additive in an effective amount to at least partially remove the catalyst fines, where the additive is an oxyalkylated acid-catalyzed alkylphenol formaldehyde resin and/or a Mannich condensate base resin copolymer.

Abstract: According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

Abstract: A method for selective extraction of lithium from geothermal brines using host-guest complexes of host molecules such as calixarene, dendrimeric polymers, hyper-branched polymers, and/or acid-catalyzed resins complexed with synergists such as organic acids, condensation polymers, olefin/maleic anhydride copolymers, and/or chelants.

Abstract: A regeneration solvent comprised of one or more ethylene amines may contact an adsorbent bed that has been used to remove sulfur compounds from a hydrocarbon stream to extract adsorbed sulfur compounds from the adsorbent material in the bed to regenerate it. The one or more ethyleneamines may have structure (I), (II), or (III): where R1, R2, R5 and R6 are, to the extent chemically possible, independently H, C1-C4 linear or branched alkyl, amido (RRNC?O), or hydroxyalkyl, where each R in the amido group is independently H or C1 alkyl, where R3 and R4 are alkylene of from 1 to 4 carbon atoms, where x ranges from 0 to 3, y ranges from 1 to 6. The regenerated adsorbent bed may be reused, either alone or in combination with a liquid-liquid extraction column, to remove sulfur compounds from a hydrocarbon stream.

Abstract: A database proxy includes a computing device and a hardware-accelerated database proxy module. The computing device and the database proxy module. The database proxy is configured to receive a write request from a client; store the write request in a commit log in a first non-volatile memory device; in response to storing the write request in the commit log, return to the client a signal acknowledging success of the write request; store the write request in a cache in a second non-volatile memory device; cause the write request to be written in a database store; and based on a first determination that the write request is stored in the cache and on a second determination that the write request is written in the database store, remove the write request from the commit log.

Abstract: The present disclosure relates to medicine delivery devices, as well as methods of delivering an aerosol medicine to a subject in need thereof. A benefit to the medicine delivery devices and methods herein can be the efficient delivery of an aerosol medicine to a subject in need thereof, thus helping to improve treatment outcomes, as well as avoiding wastage of expensive medicines. Additional benefits to the medicine delivery devices disclosed herein can be low cost, lightweight, compact, versatile, and simple to use devices useful for a wide range of patients and healthcare settings. Another benefit of the medicine delivery devices can be safer to use devices that can lower the risk of infection for patients and healthcare providers.

Abstract: The present disclosure relates to methods for treating a respiratory system of a subject, as well as medicine delivery devices for delivering an aerosol medicine to a subject in need thereof. A benefit to the methods herein can be generating a respiratory pattern of a subject that can accurately measure inhalation and exhalation patterns, which can in turn provide a benefit of more efficient and timely delivery of an aerosol medicine to a subject in need thereof. Additional benefits to the methods and devices herein can be helping to improve treatment outcomes, as well as avoiding wastage of expensive medicines. Additional benefits to the medicine delivery devices disclosed herein can be non-invasive, low cost, lightweight, compact, versatile, and simple to use devices useful for a wide range of patients and healthcare settings. Another benefit of the medicine delivery devices can be providing a single-use device that can lower the risk of infection for patients and healthcare providers.

Abstract: The present methods use a variational autoencoder (VAE) and deep generative modelling to learn meaningful representations from the immune repertoires. The system can map input sequences into a lower-dimensional latent space, which reveals a large amount of convergent sequence patterns. The system can identify patterns present in convergent clusters that are highly predictive for antigen exposure and/or antigen specificity. The system can generate, from the latent space, novel functional antibody sequence variants in-silico.

Abstract: The present disclosure describes systems and methods to make predictions classifying one or more properties of a binding protein such as an antibody, for example, antibody affinity or specificity for an antigen. The system can include one or more machine learning models that can extrapolate complex relationships between amino acid sequence and function. The system can be trained on high-quality training data generated through a two-step single-site and combinatorial deep mutational scanning approach. The trained models can then make predictions on novel variant sequences generated in silico. The present disclosure describes amino acid sequences generated by the systems and methods provided, and uses of the generated sequences to produce proteins for therapeutic and diagnostic use.

Abstract: A solvent comprised of (1) a caustic and an alcohol, (2) a caustic and a quaternary ammonium hydroxide, or (3) a caustic, an alcohol, and a quaternary ammonium hydroxide may contact an adsorbent bed that has been used to remove sulfur compounds from a hydrocarbon stream to extract adsorbed sulfur compounds from the adsorbent material in the bed to regenerate it. The regenerated adsorbent bed may be reused, either alone or in combination with a liquid-liquid extraction column, to remove sulfur compounds from a hydrocarbon stream.

Abstract: A method for manufacturing a self-disinfecting glove, the method comprising dissolving an elastomer in a solvent to form a solution, wherein the elastomer is selected from the group consisting of synthetic rubber, natural rubber, and nitrile. Adding of a photosensitizer to the elastomer solvent solution and evaporating the solvent solution to form a film, wherein the photosensitizer is selected from the group consisting of methylene blue, hypericin and titanium dioxide. Next hot pressing of the film above elastomer's melting point to form a sheet. Finally cutting, stacking and hot pressing the sheet again to enable even spread of the photosensitizer throughout the sheet.

Abstract: An extraction solvent comprised of one or more ethyleneamines having structure (I), (II), or (III): where R1-R6 can independently be H, C1-C4 linear or branched alkyl, amido (RRNC?O), or hydroxyalkyl, where each R in the amido group independently H or C1 alkyl, and where x ranges from 1 to 6, may be contacted with a hydrocarbon stream to remove or extract non-acidic contaminants, such as thiophenes, benzothiophenes, alkyl sulfides, alkyl disulfides, mercaptans, aromatics, oxygenates, metals, olefins, and combinations thereof, from the hydrocarbon stream. The extraction solvent may include co-solvents and the hydrocarbon stream may be in gas and/or liquid form.

Abstract: A regeneration solvent comprised of one or more ethylene amines may contact an adsorbent bed that has been used to remove sulfur compounds from a hydrocarbon stream to extract adsorbed sulfur compounds from the adsorbent material in the bed to regenerate it. The one or more ethyleneamines may have structure (I), (II), or (III): where R1, R2, R5 and R6 are, to the extent chemically possible, independently H, C1-C4 linear or branched alkyl, amido (RRNC?O), or hydroxyalkyl, where each R in the amido group is independently H or C1 alkyl, where R3 and R4 are alkylene of from 1 to 4 carbon atoms, where x ranges from 0 to 3, y ranges from 1 to 6. The regenerated adsorbent bed may be reused, either alone or in combination with a liquid-liquid extraction column, to remove sulfur compounds from a hydrocarbon stream.