Abstract: This present disclosure provides a method for preparing chondroitin sulfate from tilapia skull, including enzymatic hydrolyzing tilapia skull powder by Savinase 16L to obtain Savinase 16L enzymatic hydrolysate, re-enzymatic hydrolyzing the Savinase 16L enzymatic hydrolysate by 2709 alkaline protease, then separating and purificating the solution by ethanol and cetylpyridine chloride to obtain the chondroitin sulfate. The present disclosure adopts a compound enzymatic hydrolyzing method by Savinase 16L and 2709 alkaline protease to effectively achieve the efficient extraction of chondroitin sulfate of tilapia skull powder, and further achieve the purification of chondroitin sulfate by ethanol and cetylpyridine chloride, so that tilapia by-products can be utilized with high value.

Abstract: An application of Bacillus velezensis HL-5 capable of improving an extraction rate of heparin in aquatic products, the Bacillus velezensis HL-5 is preserved in Guangdong Microbial Culture Collection Center on Jul. 2, 2018, with a preservation number of GDMCC No.: 60403, which is applied to extract heparin from shellfish. Compared with a conventional enzymolysis method, the extraction rate of the heparin is increased by 10.55%-72.07%. The Bacillus velezensis HL-5 has a stronger capability of producing lipase and protease, and has a good application prospect in the extraction of the heparin of aquatic products.

Abstract: The present disclosure provides an application of fish swim bladder-derived heparin-like mucopolysaccharide in the preparation of angiogenesis inhibitors, belonging to the technical field of medication. In the present disclosure, the structural unit of the fish swim bladder-derived heparin-like mucopolysaccharide is ?-?GlcUA-[1?3]-GalNAc-4S. The fish swim bladder-derived heparin-like mucopolysaccharide has strong inhibition on angiogenesis. As shown from the results of examples in the present disclosure, the inhibitory rate of 400 mg/L fish swim bladder-derived heparin-like mucopolysaccharide on the growth of human umbilical vein endothelial cells can be up to 90.3%; and the inhibitory rate of 1 mg/mL fish swim bladder-derived heparin-like mucopolysaccharide on the angiogenesis of chick embryo chorioallantoic membrane is 77.15%.