Patents by Inventor Sakayu Shimizu

Sakayu Shimizu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190218579
    Abstract: The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.
    Type: Application
    Filed: March 29, 2019
    Publication date: July 18, 2019
    Applicants: KYOTO UNIVERSITY, NITTO PHARMACEUTICAL INDUSTRIES, LTD.
    Inventors: Jun OGAWA, Shigenobu KISHINO, Sakayu SHIMIZU, Yasunori YONEJIMA
  • Patent number: 10294501
    Abstract: The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: May 21, 2019
    Assignees: KYOTO UNIVERSITY, NITTO PHARMACEUTICAL INDUSTRIES, LTD.
    Inventors: Jun Ogawa, Shigenobu Kishino, Sakayu Shimizu, Yasunori Yonejima
  • Patent number: 10201514
    Abstract: There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: February 12, 2019
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Kengo Akimoto, Hiroshi Kawashima, Sakayu Shimizu
  • Publication number: 20170369909
    Abstract: Provided is ?3 desaturase having high enzymatic activity even at normal temperature. A polypeptide which consists of an amino acid sequence having an identity of 80% or more with the amino acid sequence represented by SEQ ID NO: 2 and has ?3 desaturation activity on C20 fatty acid, and a gene thereof.
    Type: Application
    Filed: December 24, 2015
    Publication date: December 28, 2017
    Applicants: KYOTO UNIVERSITY, NISSHIN PHARMA INC.
    Inventors: Jun OGAWA, Akinori ANDO, Eiji SAKURADANI, Sakayu SHIMIZU, Shigeru HIRAMOTO, Masataka HARATA, Yuki TAKEMOTO
  • Publication number: 20170335355
    Abstract: The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.
    Type: Application
    Filed: June 21, 2017
    Publication date: November 23, 2017
    Applicants: KYOTO UNIVERSITY, NITTO PHARMACEUTICAL INDUSTRIES, LTD.
    Inventors: Jun OGAWA, Shigenobu KISHINO, Sakayu SHIMIZU, Yasunori YONEJIMA
  • Patent number: 9782378
    Abstract: There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected.
    Type: Grant
    Filed: December 1, 2006
    Date of Patent: October 10, 2017
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Kengo Akimoto, Hiroshi Kawashima, Sakayu Shimizu
  • Patent number: 9719115
    Abstract: The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.
    Type: Grant
    Filed: November 6, 2012
    Date of Patent: August 1, 2017
    Assignees: Kyoto University, Nitto Pharmaceutical Industries, Ltd.
    Inventors: Jun Ogawa, Shigenobu Kishino, Sakayu Shimizu, Yasunori Yonejima
  • Patent number: 9493798
    Abstract: An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3?-ol content.
    Type: Grant
    Filed: July 10, 2006
    Date of Patent: November 15, 2016
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Kenichi Higashiyama, Kengo Akimoto, Sakayu Shimizu
  • Patent number: 9464305
    Abstract: An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3?-ol content.
    Type: Grant
    Filed: November 28, 2005
    Date of Patent: October 11, 2016
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Kenichi Higashiyama, Kengo Akimoto, Sakayu Shimizu
  • Publication number: 20160208297
    Abstract: Provided is an ?3 desaturase having a high enzymatic activity even at normal temperature. A polypeptide consisting of an amino acid sequence having an identity of 80% or more with the amino acid sequence represented by SEQ ID NO: 2 and having ?3 desaturation activity, and a gene therefor.
    Type: Application
    Filed: August 26, 2014
    Publication date: July 21, 2016
    Applicants: KYOTO UNIVERSITY, NISSHIN PHARMA INC.
    Inventors: Jun OGAWA, Eiji SAKURADANI, Akinori ANDO, Sakayu SHIMIZU, Masataka HARATA, Shigeru HIRAMOTO
  • Publication number: 20160175273
    Abstract: There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected.
    Type: Application
    Filed: March 2, 2016
    Publication date: June 23, 2016
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Kengo AKIMOTO, Hiroshi KAWASHIMA, Sakayu SHIMIZU
  • Patent number: 9051590
    Abstract: The present invention provides a novel acetyl-CoA carboxylase. The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.
    Type: Grant
    Filed: March 17, 2010
    Date of Patent: June 9, 2015
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Misa Ochiai, Eiji Sakuradani, Sakayu Shimizu, Jun Ogawa
  • Publication number: 20150125911
    Abstract: The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.
    Type: Application
    Filed: November 6, 2012
    Publication date: May 7, 2015
    Inventors: Jun Ogawa, Shigenobu Kishino, Sakayu Shimizu, Yasunori Yonejima
  • Patent number: 8841097
    Abstract: An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3?-ol content.
    Type: Grant
    Filed: April 21, 2006
    Date of Patent: September 23, 2014
    Assignee: Suntory Holdings Limited
    Inventors: Kenichi Higashiyama, Kengo Akimoto, Sakayu Shimizu
  • Patent number: 8367381
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: February 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Patent number: 8367382
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: February 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Publication number: 20120264960
    Abstract: The present invention provides a novel acetyl-CoA carboxylase. The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.
    Type: Application
    Filed: March 17, 2010
    Publication date: October 18, 2012
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Misa Ochiai, Eiji Sakuradani, Sakayu Shimizu, Jun Ogawa
  • Patent number: 8273562
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: September 25, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20120115203
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 10, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Publication number: 20120107883
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 3, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi