Abstract: An information processing device according to an aspect of the present disclosure includes a simulated image generation unit (131a) that generates a simulated image by superimposing a non-stained image including an autofluorescence component and a dye tile image in which a reference spectrum of a first fluorescent dye and imaging noise of each of pixels of the non-stained image are associated, a fluorescence separation unit (131b) that separates a component of the first fluorescent dye and the autofluorescence component on the basis of the simulated image and generates a separated image, and an evaluation unit (131c) that evaluates a degree of separation of the separated image.

Abstract: Provided is an advantageous technique for determining a positive threshold value for use in analyzing a stained specimen fluorescence spectrum. An information processing apparatus includes a threshold value determination unit configured to determine a positive threshold value that is to be compared with image data of a plurality of image sections included in a stained fluorescence component image, in which the positive threshold value is a criterion for determining whether or not each of the plurality of image sections corresponds to a positive cell image. The threshold value determination unit determines the positive threshold value on the basis of an unstained fluorescence component image.

Abstract: There are provided an information processing method, an information processing device, and a program capable of displaying an image in a more appropriate dynamic range. The information processing method includes: a storage step of storing first image data of a unit region image, a unit region being each region obtained by dividing a fluorescence image into a plurality of regions, and a first value indicating a predetermined pixel value range for each piece of the first image data in association with each other; and a conversion step of converting a pixel value of a combination image of a combination of the unit region images that have been selected on the basis of a representative value selected from among the first values associated with the unit region images of the combination of the unit region images that have been selected.

Abstract: It is possible to more appropriately perform fluorescence separation.

Abstract: It is possible to more appropriately perform fluorescence separation.

Abstract: It is possible to more appropriately perform fluorescence separation.