Abstract: A method for treating biomaterial is provided in which a biological tissue, typically after being cross-linked, is contacted with an anticalcification treatment solution under condition effective to render the biomaterial resistant to in vivo calcification upon implantation in a host animal. The anticalcification treatment solutions comprise higher alcohol solutions, a polyol solutions and/or a polar aprotic organic solvent solutions. Methods of reducing cytotoxicity to host tissue of bioprostheses that comprise fixed animal tissues, and treatments to reduce viral contamination of implantable medical devices are disclosed herein.