Abstract: The present disclosure relates to compositions and methods for culturing a population of hepatocytes in vitro, comprising co-culturing the population of hepatocytes with at least one non-parenchymal cell population and incubating the co-culture in culture medium, wherein the co-culture is periodically incubated in culture medium that does not comprise serum (serum-free culture medium).

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Abstract: The present disclosure relates to compositions and methods for culturing a population of hepatocytes in vitro, comprising co-culturing the population of hepatocytes with at least one non-parenchymal cell population and incubating the co-culture in culture medium, wherein the co-culture is periodically incubated in culture medium that does not comprise serum (serum-free culture medium).

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Abstract: The present disclosure provides a culture medium formulation comprising human serum that can enhance functions of primary human hepatocytes, improve morphology, promote bile canaliculi formation and extend hepatocyte functional lifetime in vitro for over 10 weeks as compared to ˜3-4 weeks when using a conventional culture medium containing serum from bovine sources. The provided long-term culture model can be used to screen drugs for their efficacious and/or toxic effects over several weeks, improve drug-transporter assays via the larger bile canaliculi network, and to model several chronic liver diseases such as hepatitis, type 2 diabetes, malaria, liver fibrosis, liver cancer, and fatty liver disease.

Abstract: The disclosure relates to in vitro cultures of human hepatocytes, and in particular co-cultures systems including human hepatocytes, non-parenchymal cells, and human endothelial cells, and the use of the co-cultures in developing and screening drugs.

Abstract: The present disclosure provides co-cultures of a population of hepatocytes, at least one non-parenchymal cell population, and a population of hepatic stellate cells in vitro, methods of preparing the co-cultures, and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The hepatocytes co-culture system provides an in vitro model in which both cell viability and phenotype are maintained for extended periods relative to conventional monoculture.

Abstract: This invention relates to methods to stabilize and/or improve the function of parenchymal cells. Also provided are systems of co-cultures of hepatocyte-stabilizing non-parenchymal cells used in bioreactor microenvironments to identify hepatic stabilizing factors by gene-expression profiling.

Abstract: The disclosure provides an in vitro culture systems. The invention provides methods and systems useful for developing in vitro an engineered tissue, method of using the tissue and compositions comprising the tissue.

Abstract: Cell cultures are provided that include a population of micropatterened hepatocytes and one or more non-parenchymal cell populations, where the hepatocytes are infected with a virus or parasite and include a reporter of virus or parasite infection. Methods of making and using the cell cultures are also provided.

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Abstract: This invention relates to methods to stabilize and/or improve the function of parenchymal cells. Also provided are systems of co-cultures of hepatocyte-stabilizing non-parenchymal cells used in bioreactor microenvironments to identify hepatic stabilizing factors by gene-expression profiling.

Abstract: The present disclosure provides a human hepatocyte co-culture which maintains the phenotype of cells from human donors with diabetes, and methods of using same. The hepatocyte co-culture system provides an in vitro model in which both cell viability and phenotype are maintained for extended periods relative to primary human hepatocyte monocultures, and is used in methods for high throughput screening and evaluation of drug candidates, and kits for performing such testing.

Abstract: This invention relates to methods to stabilize and/or improve the function of parenchymal cells. Also provided are systems of co-cultures of hepatocyte-stabilizing non-parenchymal cells used in bioreactor microenvironments to identify hepatic stabilizing factors by gene-expression profiling.

Abstract: This invention relates to methods to stabilize and/or improve the function of parenchymal cells. Also provided are systems of co-cultures of hepatocyte-stabilizing non-parenchymal cells used in bioreactor microenvironments to identify hepatic stabilizing factors by gene-expression profiling.