Abstract: The invention provides a method for the extraction of an organic acid (10) from a mixture (110), the method comprising: an extraction stage comprising providing the mixture (110) to a sorbent (150); an elution stage comprising passing an eluent along the sorbent (120) to provide an eluate (140), wherein the eluent (130) comprises a solvent selected from the group consisting of acetone and alcohols, and wherein the eluent (130) comprises at least 0.1 M of OH—, and wherein the eluent (130) comprises at least 0.1 M of an cation selected from the group comprising Na+, K+, Ca2+, Mg2+ and NH4+; a precipitation stage comprising providing CO2 (150) to the eluate (140), wherein the CO2 (150) and the cation form a carbonate, wherein the carbonate precipitates; and a separation stage comprising separating the organic acid (10) from the eluate (140).

Abstract: A method for recovering concentrated nicotine from tobacco curing includes collecting a first aqueous solution including moisture released from tobacco during curing tobacco and including nicotine. The first aqueous solution is passed through a strong cation resin to sorb the nicotine. A second aqueous solution comprising ammonium hydroxide is passed through the resin to elute the nicotine and form a third aqueous solution comprising nicotine and ammonium hydroxide. The ammonium hydroxide is removed from the third aqueous solution to generate a fourth aqueous solution comprising nicotine. The fourth aqueous solution is incubated under conditions to cause the fourth aqueous solution to separate into a fifth aqueous solution comprising a first concentration of nicotine and a sixth aqueous solution comprising a second concentration of nicotine. The first concentration of nicotine is greater than the second concentration of nicotine.

Abstract: The present disclosure is related to a method for manufacturing slabs of artificial agglomerated stone comprising: depositing a first layer (1.1) of a first mixture (M1) onto a surface (2), wherein the first layer having a first thickness h1, creating at least one cavity (3), having a width wi and a length Li, in the first layer (1.1) of first mixture (M1), depositing a second mixture (M2) into the at least one cavity (3) of the first layer (1.1), forming a second layer (1.2) by depositing the first and second mixtures, and the second layer having a second thickness h2, compacting and hardening the second layer (1.2), wherein the method further comprises after step c) and before step d), inserting a first tool (5) at least partially into the second thickness h2 of the second layer (1.2), and actuating the first tool (5) wherein the first tool (5) is configured to stir the first wall portion (4.1) while not stirring the second wall portion (4.2).

Abstract: Techniques are described for capturing data from a digital document through the use of targeted user feedback regarding the position(s) of particular data field(s) in the document. Implementations enhance the speed and accuracy of data capture from documents and/or images of documents by providing a helpful and intuitive set of controls to enable the end user to indicate the position(s) of field(s) on a document. Through specification of the position of the field in the document, implementations may isolate the portion of the document where data extraction is to be performed to determine the associated value of the field. Any suitable number of field values may be extracted from the document in this manner, based on positions indicated by the user using the provided control(s). In some implementations, one or more actions may be automatically performed based on the extracted data, such as automated bill payment actions.

Abstract: A portable multifunction personal electronic device electronically connects to one or more second electronic devices to aid in the ease of use of the one or more second electronic devices. The portable multifunction personal electronic device can be positioned as to be easily accessible to the user while the one or more second electronic devices can be stored. An optional ear piece can be electronically connected to the portable multifunction personal electronic device. The portable multifunction personal electronic device can be attached to a variety of clips to aid in attachment to a desired surface in a desired position. A method of using the portable multifunction personal electronic device rests the device against or attaches the device to a surface for ease of use.

Abstract: A method to generate configuration data to enable and/or to enhance real-time communication in a cyber-physical system or in a cyber-physical system of systems. The system includes components connected to each other by a communication infrastructure. The components each execute at least one application, which applications exchange information with at least one application being executed on another component. The components are configured to send and/or receive said information according to configuration data: The first configuration data for two or more of the components, on each of which at least one application is executed, is generated by execution of a publish-subscribe protocol, which is executed by two or more of the components, for which the first configuration data are provided.

Abstract: Techniques are described for capturing data from a digital document through the use of targeted user feedback regarding the position(s) of particular data field(s) in the document. Implementations enhance the speed and accuracy of data capture from documents and/or images of documents by providing a helpful and intuitive set of controls to enable the end user to indicate the position(s) of field(s) on a document. Through specification of the position of the field in the document, implementations may isolate the portion of the document where data extraction is to be performed to determine the associated value of the field. Any suitable number of field values may be extracted from the document in this manner, based on positions indicated by the user using the provided control(s). In some implementations, one or more actions may be automatically performed based on the extracted data, such as automated bill payment actions.

Abstract: A method to generate configuration data to enable and/or to enhance real-time communication in a cyber-physical system or in a cyber-physical system of systems. The system includes components connected to each other by a communication infrastructure. The components each execute at least one application, which applications exchange information with at least one application being executed on another component. The components are configured to send and/or receive said information according to configuration data: The first configuration data for two or more of the components, on each of which at least one application is executed, is generated by execution of a publish-subscribe protocol, which is executed by two or more of the components, for which the first configuration data are provided.

Abstract: Techniques are described for migrating information (e.g., funds) from a first account to a second account. Account information for the first account may be analyzed to identify periodic, regular, and/or semi-regular transactions of a first type (e.g., charge(s)) made from the first account. The account information may also be analyzed to identify periodic, regular, and/or semi-regular transactions of a second type (e.g., payment(s)) made into the first account. The transactions(s) of the first type may be migrated to be paid out of the second account, and the transactions(s) of the second type may be migrated to be paid into the second account. After migration of transaction(s) is completed, the remaining funds may be transferred from the first account into the second account. In some instances during the migration process, funds may be transferred from the first account to the second account to cover charges made against the second account.

Abstract: Techniques are described for capturing data from a digital document through the use of targeted user feedback regarding the position(s) of particular data field(s) in the document. Implementations enhance the speed and accuracy of data capture from documents and/or images of documents by providing a helpful and intuitive set of controls to enable the end user to indicate the position(s) of field(s) on a document. Through specification of the position of the field in the document, implementations may isolate the portion of the document where data extraction is to be performed to determine the associated value of the field. Any suitable number of field values may be extracted from the document in this manner, based on positions indicated by the user using the provided control(s). In some implementations, one or more actions may be automatically performed based on the extracted data, such as automated bill payment actions.

Abstract: A system and method for performing a marine seismic survey of a subsurface. The method includes deploying under water, from a deploying vessel, an autonomous underwater vehicle (AUV); recording with seismic sensors located on the AUV seismic waves generated by an acoustic source array; instructing the AUV to surface at a certain depth relative to the water surface; recovering the AUV by bringing the AUV on a recovery vessel; and transferring recorded seismic data to the recovery vessel.

Abstract: The potential use of these “SUMO receptors” to isolate SUMOylated targets has been considered using the SIM sequences of the SUMO-dependent ubiquitin-protein ligase RNF4. RFN4 contains 4 SIM sequences known to interact with SUMOylated proteins. The capacity of the SIM2 and SIM3 of RNF4 to purify SUMOylated proteins was increased when in the present invention it was disposed in tandem up to 4 SIM sequences. In a preferred embodiment to increase flexibility and functionality of this SUMO-trap, we have changed the natural linkers resulting in a broader capture of SUMOylated proteins. This Tandem SUMO Interacting Motifs (TSIMs) or SUMO-Binding Entities (SUBEs) system disclosed in the present invention is useful to capture polySUMOylated proteins from cell extracts. Therefore, in another embodiment of the present invention, TSIMs or SUBEs can be used for the identification SUMO substrates and the study of SUMO-regulated processes.

Abstract: System and method for monitoring a reservoir underwater. The system includes plural nodes, each having a seismic sensor for detecting seismic waves; a remote operated vehicle (ROV) configured to deploy or retrieve the plural nodes to seabed; and an autonomous underwater vehicle (AUV) configured to monitor and exchange data with the plural nodes. At least one node of the plural nodes has a head that houses the seismic sensor and the head is configured to burrow in the seabed, up to a predetermined depth, and the head remains in electrical contact through a connector with a base of the at least one node.

Abstract: The potential use of these “SUMO receptors” to isolate SUMOylated targets has been considered using the SIM sequences of the SUMO-dependent ubiquitin-protein ligase RNF4. RFN4 contains 4 SIM sequences known to interact with SUMOylated proteins. The capacity of the SIM2 and SIM3 of RNF4 to purify SUMOylated proteins was increased when in the present invention it was disposed in tandem up to 4 SIM sequences. In a preferred embodiment to increase flexibility and functionality of this SUMO-trap, we have changed the natural linkers resulting in a broader capture of SUMOylated proteins. This Tandem SUMO Interacting Motifs (TSIMs) or SUMO-Binding Entities (SUBEs) system disclosed in the present invention is useful to capture polySUMOylated proteins from cell extracts. Therefore, in another embodiment of the present invention, TSIMs or SUBEs can be used for the identification SUMO substrates and the study of SUMO-regulated processes.

Abstract: The invention relates to Tandem Ubiquitin Affinity Entities (TUBES) which are fusion polypeptides comprising at least two ubiquitin binding domains connected to each other by non-naturally occurring spacer regions. The TUBES are capable of binding with high affinity to ubiquitin and to ubiquitylated polypeptides and are therefore suitable for the purification of ubiquitylated polypeptides from cellular extracts as well as for the identification of new ubiquitylated polypeptides.

Abstract: A system and method for performing a marine seismic survey of a subsurface. The method includes deploying under water, from a deploying vessel, an autonomous underwater vehicle (AUV); recording with seismic sensors located on the AUV seismic waves generated by an acoustic source array; instructing the AUV to surface at a certain depth relative to the water surface; recovering the AUV by bringing the AUV on a recovery vessel; and transferring recorded seismic data to the recovery vessel.

Abstract: The invention relates to Tandem Ubiquitin Affinity Entities (TUBES) which are fusion polypeptides comprising at least two ubiquitin binding domains connected to each other by non-naturally occurring spacer regions. The TUBES are capable of binding with high affinity to ubiquitin and to ubiquitylated polypeptides and are therefore suitable for the purification of ubiquitylated polypeptides from cellular extracts as well as for the identification of new ubiquitylated polypeptides.