Abstract: A method provides an inter-level play billiards game. The method establishes a first set of rules for the inter-level play billiards game. Further, the first set of rules is associated with a first skill level. In addition, the method provides the first set of rules to a first player of the inter-level play billiards game. The method also establishes a second set of rules for the inter-level play billiards game. The second set of rules is distinct from the first set of rules. Further, the second set of rules is associated with a second skill level that is more experienced than the first skill level. The method provides the second set of rules to a second player of the inter-level play billiards game.

Abstract: The present invention relates to novel plasmid constructs useful for the delivery of DNA vaccines. The present invention provides novel plasmids having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The present invention, however, is particularly useful for inducing in a patient an immune response against pathogenic viruses such as HIV, measles or influenza. Immunodeficiency virus vaccine inserts of the present invention express non-infectious HIV virus-like particles (VLP) bearing multiple viral epitopes. VLPs allow presentation of the epitopes to multiple histocompatability types, thereby reducing the possibility of the targeted virus escaping the immune response. Also described are methods for immunizing a patient by delivery of a novel plasmid of the present invention to the patient for expression of the vaccine insert therein.