Abstract: The present invention relates to a method for detecting and/or enumerating a microorganism or a group of microorganisms of interest comprising a) contacting, in a container, a sample suspected of containing said microorganism or group of microorganisms with a solid growth medium containing nutrients to support growth of said microorganism or group of microorganisms and with at least one fluorescent dye; b) incubating said container under conditions and for a time sufficient to form microcolonies of said microorganism or group of microorganisms; and c) detecting and/or enumerating microcolonies emitting a fluorescent signal of said at least one fluorescent dye, thereby detecting, identifying and/or enumerating said microorganism or group of microorganisms contained in the sample.

Abstract: The present invention relates to a method, and related device, for observing, and where appropriate detecting and optionally enumerating, microbiological objects on a support, such as a membrane or a solid growth medium. In particular, said method comprises a) illuminating an area of a support with at least two incident collimated light sources forming an angle (?) of at least 10° with respect to the normal of the support, b) acquiring an image of said area of said support illuminated by said at least two incident collimated light sources by means of a light receiving element having its optical acquisition axis along the normal of the support, and c) detecting the presence or the absence of microbiological objects on said area of said support by discrimination, on said acquired image, of light reflected, scattered and/or diffused from the support and from microbiological objects on said support.

Abstract: Methods which use click-chemistry modified monosaccharide compounds of the pentose phosphate pathway, e.g. ribose, ribulose, arabinitol, xylulose, xylose or xylitol, for labeling and/or detecting an eukaryotic cell from a multicellular organism. It also relates to such modified monosaccharide compounds implemented in methods for identifying or isolating cancer cells, diagnosing a cancer or for cell therapy.

Abstract: The present invention relates to the medicinal field, in particular of oncology. It relates to a modified monosaccharide compound for use in the treatment of a cancer or more specifically tumor cancers.

Abstract: The present invention relates to modified monosaccharide compounds implemented in methods for labeling and/or detecting an eukaryotic cell from a multicellular organism. It also relates to such modified monosaccharide compounds implemented in methods for identifying or isolating cancer cells, diagnosing a cancer or for cell therapy.

Abstract: The invention relates to a method for labeling specifically living bacteria, comprising the steps of: a) incubating said bacteria of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into the envelope of such bacteria, and b) contacting said modified monosaccharide residue incorporated within the envelope of the bacteria with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group with said second reactive group, characterized in that said modified monosaccharide compound has the following formula (I), or a salt thereof: Wherein —A, B and C can be independently H, OH, NH2, OH and NH2 being substituted or not by protecting groups thereof and —D is an alkyl chain in C2 to C4, each carbon being substituted or not by OH or NH2, OH and NH2 being substituted or not b

Abstract: The invention relates to a method for labeling specifically living microorganisms in a sample comprising microorganisms, the method comprising the steps of: a) incubating said microorganisms of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into such microorganisms, and b) contacting said residue incorporated in the microorganisms, with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group of said residue incorporated within said living microorganisms with said second reactive group of said labeling molecule, resulting in a covalent link, characterized in that the said modified monosaccharide compound has the following formula (I?), or a salt thereof: —X can be O, NH or S, preferably O and NH, and —R1 and R2 can be independently H, OH, NH2, OH and NH2 bein

Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category of bacteria in a sample comprising bacteria, the method comprising the steps of: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, said monosaccharide being an endogenous monosaccharide residue of glycans of the outer membrane of such given category of bacteria, the said endogenous monosaccharide residue comprising an ulosonic acid or ulosonate salt residue, the said analog of a monosaccharide compound being a modified monosaccharide substituted at a given position by a first reactive chemical group capable to react with a second reactive group of a labeling molecule, the said given position being preferably a position which comprises a free group in the said endogenous monosaccharide residue incorporated within said glycans of the outer membrane of the bacteria, b) contacting said bacteria with a said labeling molecule comprising a said second reactive group

Abstract: A kit for labeling bacteria, the kit including: an analog of an endogenous monosaccharide residue of glycans of the outer membrane of a given category of bacteria, the endogenous monosaccharide residue being an ulosonic acid or ulosonate salt residue, the analog of a monosaccharide compound being a modified monosaccharide substituted at a given position by a first reactive group capable of reacting with a second reactive group of a labeling molecule; the labeling molecule having the second reactive group capable of reacting with said first reactive group; reactants for generating the reaction of the first reactive group of the analog residue incorporated within the glycans of the outer membrane of the bacteria with the second reactive group of the labeling molecule; and a culture or incubation medium allowing growth of the given category of bacteria, specific to the growth of the given category of bacteria.

Abstract: The invention relates to a method for labeling specifically living bacteria of a given category of Gram negative bacteria in a sample comprising bacteria, the method including the steps of: a) incubating the bacteria of the sample with at least one modified monosaccharide compound having a first reactive chemical group capable of reacting with a second reactive group, so that a monosaccharide residue bearing the first reactive group is incorporated into the polysaccharides of the outer membrane of such bacteria, and b) contacting the modified monosaccharide residue incorporated within the outer membrane of the bacteria, with a labeling molecule having a second reactive group.

Abstract: The invention relates to a method for labeling specifically living bacteria, comprising the steps of: a) incubating said bacteria of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into the envelope of such bacteria, and b) contacting said modified monosaccharide residue incorporated within the envelope of the bacteria, with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group with said second reactive group, characterized in that said modified monosaccharide compound has the following formula (I), or a salt thereof: Wherein A, B and C can be independently H, OH, NH2, OH and NH2 being substituted or not by protecting groups thereof, and D is an alkyl chain in C2 to C4, each carbon being substituted or not by OH or NH2, OH and NH2 being substituted or not b

Abstract: The invention relates to a method for labeling specifically living microorganisms in a sample comprising microorganisms, the method comprising the steps of: a) incubating said microorganisms of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into such microorganisms, and b) contacting said residue incorporated in the microorganisms, with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group of said residue incorporated within said living microorganisms with said second reactive group of said labeling molecule, resulting in a covalent link, characterized in that the said modified monosaccharide compound has the following formula (I?), or a salt thereof: —X can be O, NH or S, preferably O and NH, and —R1 and R2 can be independently H, OH, NH2, OH and NH2 bein

Abstract: A kit for labeling bacteria, the kit including: an analog of an endogenous monosaccharide residue of glycans of the outer membrane of a given category of bacteria, the endogenous monosaccharide residue being an ulosonic acid or ulosonate salt residue, the analog of a monosaccharide compound being a modified monosaccharide substituted at a given position by a first reactive group capable of reacting with a second reactive group of a labeling molecule; the labeling molecule having the second reactive group capable of reacting with said first reactive group; reactants for generating the reaction of the first reactive group of the analog residue incorporated within the glycans of the outer membrane of the bacteria with the second reactive group of the labeling molecule; and a culture or incubation medium allowing growth of the given category of bacteria, specific to the growth of the given category of bacteria.

Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category in a sample, the method including: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, and b) contacting the bacteria with a labeling molecule having a second reactive group.

Abstract: The invention relates to a method for labeling specifically living bacteria of a given category of Gram negative bacteria in a sample comprising bacteria, the method including the steps of: a) incubating the bacteria of the sample with at least one modified monosaccharide compound having a first reactive chemical group capable of reacting with a second reactive group, so that a monosaccharide residue bearing the first reactive group is incorporated into the polysaccharides of the outer membrane of such bacteria, and b) contacting the modified monosaccharide residue incorporated within the outer membrane of the bacteria, with a labeling molecule having a second reactive group.

Abstract: A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms (1) contacting said microorganisms of said sample with at least one repair compound and a growth medium, and (2) incubating the product of steps (1), and (3) detecting and quantifying said viable microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compound directly or indirectly causes an effect on the metabolism to reduce the oxidative stress of the microorganism. The invention also includes a kit for more accurately detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.

Abstract: A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms: (1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1),and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compounds comprise: (a)serine;(b)threonine;(c)a compound containing calcium ions at a dose of 10?6 to 10?2 mM;(d)a compound containing magnesium ions at a dose of 10?6 to 10?2 mM;(e)a compound containing potassium ions;(f) glutamic acid or a salt thereof; and (g)pyruvic acid or a salt thereof. The invention also discloses a kit for detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.

Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category in a sample, the method including: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, and b) contacting the bacteria with a labeling molecule having a second reactive group.

Abstract: A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms: (1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1), and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compounds comprise: (a) serine; (b) threonine; (c) a compound containing calcium ions at a dose of 10?6 to 10?2 mM; (d) a compound containing magnesium ions at a dose of 10?6 to 10?2 mM; (e) a compound containing potassium ions; (f) glutamic acid or a salt thereof; and (g) pyruvic acid or a salt thereof. The invention also discloses a kit for detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.

Abstract: A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms (1) contacting said microorganisms of said sample with at least one repair compound and a growth medium, and (2) incubating the product of steps (1), and (3) detecting and quantifying said viable microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compound directly or indirectly causes an effect on the metabolism to reduce the oxidative stress of the microorganism. The invention also includes a kit for more accurately detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.