Patents by Inventor Sam Lee Woo
Sam Lee Woo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210371919Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.Type: ApplicationFiled: March 29, 2021Publication date: December 2, 2021Inventors: Hongye SUN, Steven FUNG, Sam Lee WOO
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Patent number: 10961575Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.Type: GrantFiled: September 13, 2019Date of Patent: March 30, 2021Assignee: APPLIED BIOSYSTEMS, LLCInventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Publication number: 20200140942Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.Type: ApplicationFiled: September 13, 2019Publication date: May 7, 2020Inventors: Hongye SUN, Steven FUNG, Sam Lee WOO
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Patent number: 10415090Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.Type: GrantFiled: March 3, 2017Date of Patent: September 17, 2019Assignee: APPLIED BIOSYSTEMS, LLCInventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Publication number: 20190233887Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: December 19, 2018Publication date: August 1, 2019Inventors: Jon A. HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David M. COX, Kirk M. HIRANO, John BRIDGHAM, George Stefan GOLDA, Sam Lee WOO
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Publication number: 20170175185Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.Type: ApplicationFiled: March 3, 2017Publication date: June 22, 2017Inventors: Hongye SUN, Steven Fung, Sam Lee Woo
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Patent number: 9587277Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.Type: GrantFiled: January 18, 2016Date of Patent: March 7, 2017Assignee: Applied Biosystems, LLCInventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Publication number: 20160130650Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: ApplicationFiled: January 18, 2016Publication date: May 12, 2016Inventors: Hongye SUN, Steven FUNG, Sam Lee WOO
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Patent number: 9238835Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: GrantFiled: March 11, 2014Date of Patent: January 19, 2016Assignee: Applied Biosystems, LLCInventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Publication number: 20150087530Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: September 26, 2014Publication date: March 26, 2015Inventors: Jon A. HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David M. COX, Kirk M. HIRANO, John BRIDGHAM, George Stefan GOLDA, Sam Lee WOO
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Publication number: 20140193820Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: ApplicationFiled: March 11, 2014Publication date: July 10, 2014Applicant: Applied Biosystems, LLCInventors: Hongye SUN, Steven FUNG, Sam Lee WOO
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Publication number: 20120244537Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: ApplicationFiled: June 4, 2012Publication date: September 27, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Patent number: 8192998Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: GrantFiled: May 19, 2009Date of Patent: June 5, 2012Inventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Publication number: 20120135414Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.Type: ApplicationFiled: October 28, 2011Publication date: May 31, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Linda LEE, Sam Lee Woo, Peter Ma
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Patent number: 7897338Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.Type: GrantFiled: July 11, 2006Date of Patent: March 1, 2011Assignee: Applied Biosystems, LLCInventors: Sam Lee Woo, Ronald J. Graham, Jing Tian
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Publication number: 20090226927Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: ApplicationFiled: May 19, 2009Publication date: September 10, 2009Inventors: Hongye Sun, Steven Fung, Sam Lee Woo
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Patent number: 7129050Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.Type: GrantFiled: April 14, 2004Date of Patent: October 31, 2006Assignee: Applera CoporationInventors: Paul David Grossman, Steven Fung, Steven Michael Menchen, Sam Lee Woo, Emily Susan Winn-Deen
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Patent number: 7115376Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases.Type: GrantFiled: April 14, 2004Date of Patent: October 3, 2006Assignee: Applera CorporationInventors: Paul David Grossman, Steven Fung, Steven Michael Menchen, Sam Lee Woo, Emily Susan Winn-Deen
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Patent number: 7074569Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.Type: GrantFiled: May 28, 2004Date of Patent: July 11, 2006Assignee: Applera CorporationInventors: Sam Lee Woo, Ronald J. Graham, Jing Tian
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Patent number: 6759202Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases.Type: GrantFiled: June 10, 2002Date of Patent: July 6, 2004Assignee: Applera CorporationInventors: Paul David Grossman, Steven Fung, Steven Michael Menchen, Sam Lee Woo, Emily Susan Winn-Deen