Abstract: This invention generally relates to a novel induced cytoplasmic IVT (ICIVT) composition useful for inducing in-vitro transcription (IVT)-like RNA/mRNA amplification in the cells of interest after transfection in vitro, ex vivo and/or in vivo. The present invention is useful for designing and developing a variety of RNA/mRNA medicines as well as vaccines comprising a mixture of at least a promoter-linked RNA/mRNA-coding DNA (PLRcD) template and another DNA-dependent RNA polymerase (DdRP) mRNA sequence. Preferably, the DdRP mRNA may be selected from the mRNA of T7, T3 and/or SP6 RNA polymerase, or a combination thereof, while the PLRCD template may encode the transcripts of antisense RNA oligonucleotide (aRNA-ASO), small interferring RNA (siRNA), double-stranded RNA (dsRNA), short hairpin RNA (shRNA), microRNA (miRNA)/microRNA precursor (pre-miRNA), long noncoding RNA (lncRNA), messenger RNA (mRNA), and/or self-amplifying RNA/mRNA (saRNA/samRNA), or a combination thereof.

Abstract: This invention relates to a novel modified RNA composition (called oxo-RNA) comprising at least an oxo-nucleotide (containing oxopurine) in its 3?-end region (e.g. 3?-tail, 3?-UTR). The oxo-nucleotide includes 8-hydroxyguanine/8-oxoguanine/7,8-dihydro-8-oxoguanine (or called oxo-G/oxo-dG) and 8-hydroxyadenine/8-oxoadenine (or called oxo-A/oxo-dA). Oxo-RNA can be a single-stranded RNA sequence or double-stranded duplex, or even an RNA-DNA hybrid duplex. Advantageously, this new oxo-RNA composition not only greatly enhance RNA/DNA stability and functionality but also can prevent TREX1-mediated degradation and the related non-specific immunity over-activation (e.g. cytokine storm).

Abstract: A superabsorbent polymers and a method of forming the same are provided. The method is processed by adding calcined shell powders to a free radical polymerization. The superabsorbent polymers with more micropores can be obtained. Therefore, absorptivity and permeability for the liquid of the superabsorbent polymers are increased, and diffusibility and liquid conductivity of the superabsorbent polymers are also improved.

Abstract: This invention relates to a novel composition of RNA/mRNA medicines and/or vaccines produced by using Replicase/RNA-dependent RNA polymerase (RdRP)-mediated RNA Cycling Reaction (RCR). This RCR-amplifiable RNA/mRNA composition comprises at least a replicase/RdRP-binding site (RdRP-BS) in the 5?-end or 3?-end, or both, of a desired RNA sequence of interest, to form a self-amplifying RNA/mRNA (samRNA) platform. The samRNA platform so obtained is useful for designing and developing a variety of self-amplifying RNA/mRNA (samRNA) constructs, of which the desired RNA sequences may include, but not limited to, antisense oligonucleotide RNA (aRNA; ASO), small interfering RNA (siRNA), short hairpin RNA (shRNA), microRNA (miRNA)/miRNA precursor (pre-miRNA), long non-coding RNA (lncRNA), and/or messenger RNA (mRNA), or a combination thereof. The present RdRP-BS designs in said samRNA are derived or modified from the identified RdRP-BS motifs of coronavirus (e.g.

Abstract: This invention generally relates to a novel composition of RNA/mRNA medicines as well as vaccines produced by using replicase- and/or RNA-dependent RNA polymerase (RdRp)-mediated RNA cycling reaction (RCR). The present invention is useful for developing a variety of self-amplifying RNA/mRNA (samRNA) medicines and vaccines containing at least a replicase/RdRp-binding site in the 5?- or 3?-end, or both, of any desired RNA molecule, including but not limited to antisense RNA (aRNA), small interferring RNA (siRNA), short hairpin RNA (shRNA), microRNA (miRNA)/miRNA precursor, long non-coding RNA (lnRNA) and mRNA. These RNA molecules can be either in single-stranded or in double-stranded, or mixed, conformation. The samRNA so obtained is useful not only for producing RNA-based vaccines and/or medicines but also for generating the mRNA-associated proteins, peptides, and/or antibodies under a proper in-vitro or in-cell translation condition.

Abstract: Disclosed herein is directed to a synthetic ribonucleic acid (RNA) fragment that includes an RNA template flanked by at least of 5?-end RNA-dependent RNA polymerase (RdRp) binding site and a 3?-end RdRp binding site, thus the synthetic RNA fragment can be amplified in vitro via an RNA-dependent RNA cycling reaction (RCR). Also disclosed herein is a method for producing an amplified RNA product by use of the present synthetic RNA fragment.

Abstract: This invention relates to a novel composition and method for RNA/mRNA production as well as amplification using viral RNA replicase and/or RNA-dependent RNA polymerase (RdRp) enzymes and the use of associated RNA/mRNA products thereof. The present invention can be used for manufacturing and amplifying all varieties of RNA/mRNA sequences carrying at least a replicase/RdRp-binding site in the 5?- or 3?-end, or both. The RNA/mRNA so obtained is useful for not only producing mRNA vaccines and/or RNA-based medicines but for generating the mRNA-associated proteins, peptides, and/or antibodies under an in-vitro as well as in-cell translation condition. Principally, the present invention is a novel RNA replicase/RdRp-mediated RNA/mRNA amplification method, namely Replicase Cycling Reaction (RCR). The RNA replicases involved in RCR include but not limited to viral and/or bacteriophage RNA-dependent RNA polymerases (RdRp) in either modified or non-modified mRNA and/or protein compositions, particularly coronaviral (e.

Abstract: A superabsorbent polymers and a method of forming the same are provided. The method is processed by adding calcined shell powders to a free radical polymerization. The superabsorbent polymers with more micropores can be obtained. Therefore, absorptivity and permeability for the liquid of the superabsorbent polymers are increased, and diffusibility and liquid conductivity of the superabsorbent polymers are also improved.

Abstract: This invention generally relates to a novel RNA/mRNA production and amplification method using viral RNA replicase and/or RNA-dependent RNA polymerase (RdRp) enzymes as well as the associated mRNAs thereof. The present invention can be used for manufacturing and amplifying all varieties of RNA/mRNA sequences carrying at least an RdRp-binding site in the 5?- or 3?-end, or both. The RNA/mRNA so obtained is useful for not only producing mRNA vaccines and/or RNA-based medicines but also for generating the mRNA-associated proteins, peptides, and/or antibodies under an in-vitro as well as in-cell translation condition. Principally, the present invention is a novel RNA replicase-mediated RNA/mRNA amplification method, namely Replicase Cycling Reaction (RCR). The RNA replicases involved in RCR include but not limited to viral and/or bacteriophage RNA-dependent RNA polymerases (RdRp), particularly coronaviral and hepatitis C viral (HCV) RdRp enzymes.

Abstract: This invention generally relates to a novel RNA composition and its production method useful for generating and expanding induced pluripotent stem cells (iPS cells; iPSC) as well as adult stem cells (ASC). The RNA composition so defined can be used for producing not only non-transgenic but also tumor-free iPS cells. The defined RNA composition contans at least two types of different RNA constructs; one is “miR-302 precursor RNA (pre-miR-302)” and the other is “RNA-dependent RNA polymerase (RdRp)” mRNA. Both of pre-miR-302 and RdRp mRNA contain highly structured RNA comformations, such as hairpin and stem-loop structures. To produce highly structured RNAs, a novel PCR-IVT methodology has been developed and used with a specially designed RNA polymerase-helicase mixture activity.

Abstract: A superabsorbent polymer includes polymer particles, surface cross-linking agents and particles made of silicon-containing inorganic salt. The polymer particles have cross-linking inside the polymer particles. The surface cross-linking agents are covalently bound to the surface of the polymer particles so as to constitute a surface cross-linked region at the surface of each said resin particle, and the particles made of silicon-containing inorganic salt cover the surface of the polymer particles.

Abstract: A detachable structure for shielding input/output (I/O) ports of a motherboard includes a housing and a blocking plate. The housing has a plurality of installed portions, an internal space, and an opening, the internal space communicates with the opening, and the plurality of installed portions are arranged in the internal space. The housing is arranged on the motherboard, and the internal space accommodates the I/O ports. The blocking plate is penetrated with a plurality of first holes. A plurality of installing elements are respectively fixed to the installed portions to fix the blocking plate to the housing, thereby shielding the opening. The positions of the plurality of first holes respectively correspond to the positions of the I/O ports to use the plurality of first holes to expose the I/O ports.

Abstract: A superabsorbent polymer includes polymeric particles, surface cross-linking agents and an extract of a plant of Sapindaceae. The polymeric particles have cross-linking inside the polymeric particles. The surface cross-linking agents are covalently bound to the surface of the polymeric particles so as to constitute a layer of surface cross-linked region at the surface of each polymeric particle, and the extract of the plant of Sapindaceae covers the surface of the polymeric particles.

Abstract: A fixing device for an expansion card is installed on a frame of a desktop computer to fix an expansion card on the desktop computer. The fixing device includes at least two installation bases. After adjusting an interval between the installation bases, the installation bases are fixed to an opening of the frame by locking members. Each of the installation bases is provided with an installing through hole, such that an installation portion at the rear end of each of two fixing rods penetrates through the installing through. The front ends of the fixing rods vertically extend to form clamping portions to clamp the expansion card. The clamping portions and the expansion card are provided with a fixing batten to stabilize the expansion card.