Patents by Inventor Sam Woo

Sam Woo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250019756
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Application
    Filed: June 10, 2024
    Publication date: January 16, 2025
    Inventors: Linda LEE, Sam WOO, Peter MA
  • Publication number: 20240425916
    Abstract: The present disclosure provides systems and methods for sequencing nucleic acid molecules using tunneling labels. A sequence of a nucleic acid molecule may be identified with high accuracy using a chip comprising sensors, wherein each individual sensor may comprise at least two electrodes separated by a gap. The electrodes may be configured to generate at least one electrical signal upon binding of a tunneling label associated with a nucleotide. Epigenetic information can also be determined at the same time as a nucleic acid sequence.
    Type: Application
    Filed: August 30, 2024
    Publication date: December 26, 2024
    Inventors: Mark F. OLDHAM, Eric S. NORDMAN, Timothy M. WOUDENBERG, Gaurav GOYAL, Masoud VAKILI, Toshihiko HONKURA, Sam WOO, Hisao KAWASAKI, Kazusuke MIHARA
  • Patent number: 12091712
    Abstract: The present disclosure provides systems and methods for sequencing nucleic acid molecules using tunneling labels. A sequence of a nucleic acid molecule may be identified with high accuracy using a chip comprising sensors, wherein each individual sensor may comprise at least two electrodes separated by a gap. The electrodes may be configured to generate at least one electrical signal upon binding of a tunneling label associated with a nucleotide. Epigenetic information can also be determined at the same time as a nucleic acid sequence.
    Type: Grant
    Filed: October 24, 2018
    Date of Patent: September 17, 2024
    Assignee: Illumina Cambridge, Ltd.
    Inventors: Mark F. Oldham, Eric S. Nordman, Timothy M. Woudenberg, Gaurav Goyal, Masoud Vakili, Toshihiko Honkura, Sam Woo, Hisao Kawasaki, Kazusuke Mihara
  • Patent number: 12006536
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: August 11, 2021
    Date of Patent: June 11, 2024
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Publication number: 20220389499
    Abstract: A method for forming discrete volumes of aqueous fluid may comprise flowing aqueous fluid into a first conduit from a supply of aqueous fluid and flowing into the first conduit a spacing liquid supplied from a second conduit, the spacing liquid being immiscible with the aqueous fluid. The flowing of the aqueous fluid and the spacing liquid into the first conduit forms discrete volumes of the aqueous fluid, with consecutive discrete volumes of the aqueous fluid separated by the spacing liquid. The method may further comprise transferring the discrete volumes of the aqueous fluid and spacing liquid from the first conduit to a third conduit for processing.
    Type: Application
    Filed: April 25, 2022
    Publication date: December 8, 2022
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Willy K. Wiyatno, Linda Lee, David Cox, James Nurse, Douglas Greiner, Dennis Lehto, Janusz Wojtowicz, Sam Woo, Richard Reel
  • Patent number: 11319585
    Abstract: A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: May 3, 2022
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Willy K. Wiyatno, Linda Lee, David Cox, James Nurse, Douglas Greiner, Dennis Lehto, Janusz Wojtowicz, Sam Woo, Richard Reel
  • Publication number: 20220049289
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Application
    Filed: August 11, 2021
    Publication date: February 17, 2022
    Inventors: Linda LEE, Sam WOO, Peter MA
  • Patent number: 11104944
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: January 14, 2019
    Date of Patent: August 31, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Publication number: 20200149099
    Abstract: A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.
    Type: Application
    Filed: October 17, 2019
    Publication date: May 14, 2020
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Willy K. WIYATNO, Linda LEE, David COX, James NURSE, Douglas GREINER, Dennis LEHTO, Janusz WOJTOWICZ, Sam WOO, Richard REEL
  • Patent number: 10450604
    Abstract: A method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The discrete volumes are separated from one another by a liquid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: October 22, 2019
    Assignee: Applied Biosystems, LLC
    Inventors: Willy Wiyatno, Linda Lee, David Cox, James Nurse, Douglas Greiner, Dennis Lehto, Janusz Wojtowicz, Sam Woo, Richard Reel
  • Publication number: 20190211373
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Application
    Filed: January 14, 2019
    Publication date: July 11, 2019
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Publication number: 20190169684
    Abstract: The present disclosure provides systems and methods for sequencing nucleic acid molecules using tunneling labels. A sequence of a nucleic acid molecule may be identified with high accuracy using a chip comprising sensors, wherein each individual sensor may comprise at least two electrodes separated by a gap. The electrodes may be configured to generate at least one electrical signal upon binding of a tunneling label associated with a nucleotide. Epigenetic information can also be determined at the same time as a nucleic acid sequence.
    Type: Application
    Filed: October 24, 2018
    Publication date: June 6, 2019
    Inventors: Mark F. OLDHAM, Eric S. NORDMAN, Timothy M. WOUDENBERG, Gaurav GOYAL, Masoud VAKILI, Toshihiko HONKURA, Sam WOO, Hisao KAWASAKI, Kazusuke MIHARA
  • Patent number: 10221449
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: March 5, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Nien-Tung Ma
  • Publication number: 20180037931
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Application
    Filed: July 17, 2017
    Publication date: February 8, 2018
    Inventors: Linda LEE, Sam WOO, Peter Nien-Tung MA
  • Patent number: 9708650
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: July 18, 2017
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Publication number: 20170051342
    Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Application
    Filed: August 8, 2016
    Publication date: February 23, 2017
    Inventors: LINDA LEE, Sam WOO, Peter MA
  • Patent number: 9410192
    Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: October 14, 2013
    Date of Patent: August 9, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Publication number: 20160097087
    Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
    Type: Application
    Filed: September 3, 2015
    Publication date: April 7, 2016
    Inventors: Willy Wiyatno, Linda Lee, David Cox, James Nurse, Douglas Greiner, Dennis Lehto, Janusz Wojtowicz, Sam Woo, Richard Reel
  • Patent number: 9194772
    Abstract: Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
    Type: Grant
    Filed: September 10, 2009
    Date of Patent: November 24, 2015
    Assignee: Applied Biosystems, LLC
    Inventors: Linda Lee, Sam Woo, Congcong Ma, Richard Reel, Mark Oldham, David Cox, Benjamin Schroeder, Jon Sorenson, Willy Wiyatno
  • Patent number: 9140630
    Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: September 22, 2015
    Assignee: Applied Biosystems, LLC
    Inventors: Willy Wiyatno, Linda Lee, David Cox, Jim Nurse, Douglas Greiner, Dennis Lehto, Janusz Wojtowicz, Sam Woo, Richard Reel