Abstract: The present invention provides a method for directly measuring apolipoprotein B-100 (apoB) or cholesterol associated with very low density lipoprotein (VLDL) in a fluid sample. In one embodiment the method involves the specific capture of intact VLDL particles from a fluid sample with a specific VLDL binding agent. The quantity of VLDL-apoB present in the sample is then measured by detecting the amount of VLDL-apoB bound to the binding agent-VLDL complexes formed in the reaction. In an alternative embodiment of the method, intact VLDL particles from a fluid sample are also captured with a specific VLDL binding agent and thereafter the cholesterol associated with the bound VLDL is determined. The cholesterol contained in the binding-agent-VLDL complexes can be detected by reacting the complexes with labeled cholesterol specific binding agents and measuring the amount of label bound therto, or by releasing the cholesterol in the complexes and measuring the amount of cholesterol released.

Abstract: The present invention provides a method for directly measuring apolipoprotein B-100 (apoB) or cholesterol associated with very low density lipoprotein (VLDL) in a fluid sample. In one embodiment the method involves the specific capture of intact VLDL particles from a fluid sample with a specific VLDL binding agent. The quantity of VLDL-apoB present in the sample is then measured by detecting the amount of VLDL-apoB bound to the binding agent-VLDL complexes formed in the reaction. In an alternative embodiment of the method, intact VLDL particles from a fluid sample are also captured with a specific VLDL binding agent and thereafter the cholesterol associated with the bound VLDL is determined. The cholesterol contained in the binding-agent-VLDL complexes can be detected by reacting the complexes with labeled cholesterol specific binding agents and measuring the amount of label bound therto, or by releasing the cholesterol in the complexes and measuring the amount of cholesterol released.

Abstract: The present invention provides a method for directly measuring apolipoprotein B-100 (apoB) or cholesterol associated with very low density lipoprotein (VLDL) in a fluid sample. In one embodiment the method involves the specific capture of intact VLDL particles from a fluid sample with a specific VLDL binding agent. The quantity of VLDL-apoB present in the sample is then measured by detecting the amount of VLDL-apoB bound to the binding agent-VLDL complexes formed in the reaction. In an alternative embodiment of the method, intact VLDL particles from a fluid sample are also captured with a specific VLDL binding agent and thereafter the cholesterol associated with the bound VLDL is determined. The cholesterol contained in the binding-agent-VLDL complexes can be detected by reacting the complexes with labeled cholesterol specific binding agents and measuring the amount of label bound therto, or by releasing the cholesterol in the complexes and measuring the amount of cholesterol released.

Abstract: The present invention provides monoclonal antibodies specific for kringle 5 of apo(a) and hybridomas secreting such antibodies. The invention also relates to assay methods for directly measuring concentrations of lipoprotein(a) [Lp(a)] in a plasma sample. In one embodiment, the method involves the specific capture of Lp(a) from a plasma sample with a monoclonal antibody developed against kringle 5 of apo(a), which is non-cross-reactive with plasminogen and kringle 4 of apo(a). The quantity of the Lp(a) present in the sample is then measured by detecting the amount of Lp(a)-anti-kringle 5 complex that has formed in the reaction. Alternatively, the Lp(a) may be captured non-specifically and then detected with the monoclonal antibody specific for kringle 5 of apo(a). The invention also provides competitive assays using the above-mentioned kringle 5 specific monoclonal antibodies.

Abstract: The present invention provides monoclonal antibodies specific for kringle 5 of apo(a) and hybridomas secreting such antibodies. The invention also relates to assay methods for directly measuring concentrations of lipoprotein(a) [Lp(a)] in a plasma sample. In one embodiment, the method involves the specific capture of Lp(a) from a plasma sample with a monoclonal antibody developed against kringle 5 of apo(a), which is non-cross-reactive with plasminogen and kringle 4 of apo(a). The quantity of the Lp(a) present in the sample is then measured by detecting the amount of Lp(a)-anti-kringle 5 complex that has formed in the reaction. Alternatively, the Lp(a) may be captured non-specifically and then detected with the monoclonal antibody specific for kringle 5 of apo(a). The invention also provides competitive assays using the above-mentioned kringle 5 specific monoclonal antibodies.

Abstract: The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.

Abstract: Methods and materials detect ketone and aldehyde analytes in fluid samples by reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.

Abstract: The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.

Abstract: The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a matrix comprising a hydrazine compound coupled to an H.sup.+ ion exchange resin to produce a hydrazone reaction product with a characteristic color. Methods are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject by means of contacting said breath with a device comprising an H.sup.+ ion exchange resin to which a hydrazine compound has been coupled and correlating the breath acetone concentration to a standard reflecting the effect on breath acetone of fixed rates of fat metabolism.