Abstract: A fluidic chip for detecting an analyte is provided, which comprises (a) at least one device comprising (i) a fluid inlet and (ii) at least one fluid transport channel that passes through a detection region, wherein (1) the at least one fluid transport channel has varying cross-sectional area as a function of position along the channel length and/or (2) the device comprises two or more fluid transport channels configured with two or more different cross-sectional areas; and (b) at least one fluid outlet, wherein the device provides for transport of single molecules across the detection region at a desired duty cycle by (1) allowing for detection of an analyte at a different position along the channel length based on the desired cross-sectional area and/or (2) allowing for detection of an analyte in a specific channel configured with the desired cross-sectional area. This application also provides for method of optimizing duty cycle using fluidic chips.

Abstract: Nanofluidic chips are described herein that are configured for high-volume manufacturing and maintaining sample integrity in multiplexed devices comprising: at least two devices, wherein each device comprises at least one sample inlet and at least one nanochannel; and a detection region, wherein the at least two devices pass through the detection region and wherein the at least two devices are fluidically distinct from the inlet through the detection region, and wherein actuation energy can be applied independently to at least two devices.