Abstract: An optoacoustic probe for optoacoustic imaging of a volume is provided that includes a housing extending from a distal end operable to contact the volume to a proximal end. The optoacoustic probe includes a light source configured to generate light that is transmitted along a light pathway to generate return signals when the light reacts with the volume, and a transducer assembly including a transducer configured to receive the optoacoustic return signals and an acoustic lens provided over the transducer. The optoacoustic probe also includes a steering assembly coupled within the housing and configured to steer the light pathway to generate the light along the light pathway at different scanning areas of the volume based on the return signals.

Abstract: The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.

Abstract: A level of busyness of a public place may be represented using content specific to the public place. Images, video, audio, or other content captured at the public place is correlated with busyness information for the public place at the time of capture. Representative content is selected and stored based on a number of factors such as to minimize resources in storing and transmitting the content. The selected representative content may be provided in response to a user request for information related to the public place, such that the user receives an accurate portrayal of how the public place would look, sound, and/or feel at a given time.

Abstract: Disclosed herein are apparatuses, systems, as well as related methods, computing devices, and computer-readable media related to real time cell sorter cell sorting using embeddings. For example, in some embodiments a method may comprise receiving first cell sorter data. The first cell sorter data may include cell sorter data including microscopy data, hyperspectral imaging data, high-dimensional vector data, or one or more combinations thereof. In some embodiments, the cell sorter data may include quantitative fluorescence data expressed as one or more of antibodies bound per cell, antibody binding capacity (ABC), molecules of equivalent soluble fluorochrome (MESF), one or more other quantitative indicators of fluorescence, or one or more combinations thereof. In some embodiments, the quantitative fluorescence data includes one or more fluorescence signals from: one or more fluorescent proteins, one or more fluorescent dyes, one or more fluorescently conjugate antibodies, or one or more combinations thereof.

Abstract: Disclosed are systems, computer-program products, and computer-implemented methods for the automatic estimation of geographic boundaries. Implementations of the foregoing may be useful for automatedly determining the geographic boundary of a community. In some embodiments, a computing device may receive birth location data related to the community. Enriched birth locations for the community may be determined based on likelihood metrics. An estimated geographic boundary of the community may be determined. The estimated geographic boundary may correspond to a probability density of the enriched birth locations represented using the geographical coordinates. A graphical user interface may present a map and the estimated geographic boundary of the community overlaying the map.

Abstract: The present disclosure provides systems, kits, and methods for nucleic acid integration utilizing engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated transposon (CRISPR-Tn) system. More particularly, the present disclosure provides systems comprising: an engineered CRISPR-Tn system or one or more nucleic acids encoding the engineered CRISPR-Tn system, wherein the CRISPR-Tn system comprises at least one or both of: a) at least one Cas protein (e.g., Cas6, Cas7, Cas5, and/or Cas8); and b) one or more transposon-associated proteins (e.g., TnsA, TnsB, TnsC, TnsD, and/or TniQ). The present disclosure also provides systems, kits, and methods for nucleic acid integration in a eukaryotic cell.

Abstract: A system efficiently selects at least one device from multiple devices based on received audio signals. In some instances, the system receives audio signals from devices that each comprise at least one microphone. A respective audio signal of the audio signals includes a representation of a sound originating from a location. The system then determines a device to be used to respond to the sound. In some instances, the system analyzes times in which the received audio signals that represent the sound are generated and/or volumes of the sound as represented by the received audio signals. The system can then select the device based on the analysis.

Abstract: The present disclosure provides systems, kits, and methods provide systems and methods for recruiting one or more effector domains to a target nucleic acid and or modulating expression of a target gene in a cell utilizing an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) system. More particularly, the present disclosure provides systems comprising: an engineered CRISPR-Cas system or one or more nucleic acids encoding the engineered CRISPR-Cas system, wherein the CRISPR-Cas system comprises: at least one Cas protein (e.g., Cas6, Cas7, Cas5, Cas8 and/or Cas12k); a guide RNA (gRNA) complementary to at least a portion of the target nucleic acid sequence; and, optionally, at least one transposon-associated protein (e.g., TniQ, TnsC, TnsA, and/or TnsB).

Abstract: A method of providing an instructional scaffold to person learning to read English language comprises displaying printed matter to the learner in the form of lists. Preferably text stories. The normal spacing of the letters in words is kept intact; in a first part of the method the rime portions of monosyllable words are bolded and made larger than the onset portions; and in a second part of the method, which also may be used independently, the sequential syllables of multisyllable words are emphasized by alternating plain font with bolded font and the long vowels are identified, for example by underscore, again with the words of any text being kept intact.

Abstract: One of the most challenging problems that marketing professionals face is measuring the effect of advertising campaigns on the sales of a product. One of the main causes of poorly allocated spend is incorrect analysis of advertising campaign effectiveness. Introduced here is an approach to determining advertising campaign effectiveness in a more accurate, dependable manner using machine learning to extract the true effect of an advertising. This approach can be implemented by a data analysis platform that is able to train a machine learning algorithm using company-specific data as part of a training operation, as well as implementing the resulting machine learning model as part of an inferencing operation.

Abstract: An optoacoustic probe for optoacoustic imaging of a volume is provided that includes a housing extending from a distal end operable to contact the volume to a proximal end. The optoacoustic probe includes a light source configured to generate light that is transmitted along a light pathway to generate return signals when the light reacts with the volume, and a transducer assembly including a transducer configured to receive the optoacoustic return signals and an acoustic lens provided over the transducer. The optoacoustic probe also includes a steering assembly coupled within the housing and configured to steer the light pathway to generate the light along the light pathway at different scanning areas of the volume based on the return signals.

Abstract: The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.

Abstract: The present disclosure provides methods and systems for resolving cointegrate products from RNA-guided DNA integration. More particularly, the present disclosure provides systems comprising: an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) (CRISPR-Cas) system, and/or one or more vectors encoding the engineered CRISPR-Cas system, wherein the CRISPR-Cas system comprises: (a) at least one Cas protein, and (b) a guide RNA (gRNA); an engineered transposon system, and/or one or more vectors encoding the engineered transposon system, wherein the transposon system is configured for replicative transposition; a recombinase, or catalytic domain thereof, and/or one or more vectors encoding a recombinase, or catalytic domain thereof; and at least one donor nucleic acid to be integrated, wherein the donor nucleic acid comprises a recognition site for the recombinase and a cargo nucleic acid flanked by at least one transposon end sequence, and methods of use thereof.

Abstract: An apparatus (10) for coupling with a pipe (12), the apparatus (10) including a body assembly (13) having a body (14) adapted to fit with the pipe (12), and at least two gripping parts (16) moveably coupled to the body (14), the at least two gripping parts (16) being adapted to be moveable between an insertion condition, in which the gripping parts (16) are resiliently depressible to allow movement of the pipe (12) in a first insertion direction, and a gripping condition in which the gripping parts (16) each engage with opposing side walls (18) of the pipe (12) to restrict movement of the pipe (12) in a second opposing direction thereby coupling the apparatus (10) and the pipe (12).

Abstract: A system efficiently selects at least one device from multiple devices based on received audio signals. In some instances, the system receives audio signals from devices that each comprise at least one microphone. A respective audio signal of the audio signals includes a representation of a sound originating from a location. The system then determines a device to be used to respond to the sound. In some instances, the system analyzes times in which the received audio signals that represent the sound are generated and/or volumes of the sound as represented by the received audio signals. The system can then select the device based on the analysis.

Abstract: A laser assembly is provided that includes a laser pump chamber that emits a first light through a laser cavity at a first wavelength, and a polarization assembly that receives the first light and converts the first light into a pulsed light that includes the first light at the first wavelength and a first polarization. The laser assembly also includes a second light at the first wavelength and a second polarization.

Abstract: A system, method and computer program for predictive maintenance on natural gas compressors through auditory detection. Using one or multiple microphones, a system will collect and evaluate sound waves for the purpose of predicting and detecting failures and alert conditions in mechanical and process equipment. The system will collect sound which is used in a machine learning environment to utilize supervised training as well as unsupervised training, to produce a normal baseline and detect abnormal operations. Additionally, abnormal operations are categorized against known conditions. For uncategorized and unknown conditions, a workflow is in place to allow for the retraining and learning” of new conditions which are then published to the entire network of devices.

Abstract: The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.

Abstract: In certain embodiments, the present systems and methods use Tn7-like transposons that encode CRISPR-Cas systems for programmable, RNA-guided DNA integration. For example, the CRISPR-Cas machinery directs the Tn7 transposon-associated proteins to integrate DNA downstream of a target site (e.g., a genomic target site) recognized by a guide RNA (gRNA).

Abstract: The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.