Abstract: The present disclosure provides systems and methods for targeted nucleic acid deletions and inactivation of a gene of interest comprising a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) (CRISPR-Cas) system comprising at least one Cas protein and a pair of guide RNAs (gRNAs), an engineered transposon system, at least one donor nucleic acid, and a recombinase.

Abstract: The present disclosure provides devices, systems, and methods for detection of nucleic acids based on CRISPR-Cas editing systems, for example for use in biosurveillance. Disclosed herein are systems and methods utilizing two devices: 1) a point of—need disposable “FET Strip” (enzymatic), and 2) an instrument-operated “FET Multiplexor” (electronic), to provide detection of a nucleic acid for biosurveillance.