Abstract: The invention provides bidirectional expression vectors comprising Chinese hamster ovary elongation factor 1-? (CHEF1) transcriptional regulatory DNA elements, a gene of interest (GOI), a minimal cytomegalovirus (minCMV) and a selectable marker (SM) and/or a human adenovirus tripartite leader (AdTPL) sequence. The invention also provides method for increasing heterologous protein expression in a host cell comprising culturing the host cell the bidirectional expression vector(s).

Abstract: Described herein are polynucleotide constructs and stable cell lines for inducible production of rAAV virions within which are packaged a payload polynucleotide.

Abstract: The present disclosure provides polynucleotides, vector systems, cells, and methods for amplifying expression of proteins, such as AAV Rep and Cap proteins or therapeutic proteins (e.g., an antibody). Increased expression of AAV Rep and Cap proteins is useful in increasing production of recombinant AAV virions. Also provided herein are vector systems for inducing amplification of expression of AAV Rep and Cap proteins. Also provided herein are vector systems for inducing amplification of expression of therapeutic proteins. Inducible expression of therapeutic proteins is useful in increasing production of therapeutic proteins.

Abstract: Described herein are polynucleotide constructs for inducible production of rAAV virions. Also provided are stable cell lines for inducible expression of rAAV virions that include a pay load poly nucleotide.

Abstract: Provided are compositions and methods for silencing suppressor tRNAs. Further provided are compositions and methods for silencing suppressor tRNAs during AAV production for producing virions comprising sequences coding for suppressor tRNAs.

Abstract: Described herein are compositions and methods of using stable cells lines in combination with transient transfection to produce recombinant AAV (rAAV).

Abstract: Described herein are polynucleotide constructs and stable cell lines for inducible production of rAAV virions within which are packaged a payload polynucleotide.

Abstract: Disclosed herein are cells and cell lines that are selected for retention of at least two exogenous nucleic acid constructs using a single selective pressure. Also disclosed herein are compositions and methods for generating recombinant cells and cell lines using a single selective pressure.

Abstract: Described herein are polynucleotide constructs and stable cell lines for inducible production of rAAV virions within which are packaged a payload polynucleotide.

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell-cycle inhibitors to growing cell cultures.

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell-cycle inhibitors to growing cell cultures.

Abstract: Methods of modulating high mannose glycoform content of a protein in a cell culture by contacting the cells expressing the protein of interest with monesin are provided.

Abstract: The invention provides bidirectional expression vectors comprising Chinese hamster ovary elongation factor 1-a (CHEF1) transcriptional regulatory DNA elements, a gene of interest (GO I), a minimal cytomegalovirus (minCMV) and a selectable marker (SM) and/or a human adenovirus tripartite leader (AdTPL) sequence. The invention also provides method for increasing heterologous protein expression in a host cell comprising culturing the host cell the bidirectional expression vector(s).

Abstract: Methods of modulating high mannose glycoform content of a protein in a cell culture by contacting the cells expressing the protein of interest with monesin are provided.