Abstract: The present invention provides methods for utilizing blended compositions of acetonitrile and toluene as organic wash solvents in the production of a synthetic oligonucleotide, the blended compositions producing higher synthetic oligonucleotide yields than a pure acetonitrile wash solvent. The method also provides a process for removing one or more impurities from the acetonitrile and toluene containing wash solvent received as a waste stream from the oligonucleotide synthesis process. The process includes adding at least one of an iodine reactive compound, a sulfur reactive compound and/or an acidic reactive compound to the waste stream, and fractionating the waste stream. The fractionation produces an overhead fraction and a bottom fraction where the overhead fraction includes the acetonitrile and the toluene, and the bottom fraction includes the one or more impurities.

Abstract: The present invention provides methods for utilizing blended compositions of acetonitrile and toluene as organic wash solvents in the production of a synthetic oligonucleotide, the blended compositions producing higher synthetic oligonucleotide yields than a pure acetonitrile wash solvent. The method also provides a process for removing one or more impurities from the acetonitrile and toluene containing wash solvent received as a waste stream from the oligonucleotide synthesis process. The process includes adding at least one of an iodine reactive compound, a sulfur reactive compound and/or an acidic reactive compound to the waste stream, and fractionating the waste stream. The fractionation produces an overhead fraction and a bottom fraction where the overhead fraction includes the acetonitrile and the toluene, and the bottom fraction includes the one or more impurities.

Abstract: According to the present invention, there is provided a process for making an oligonucleotide or a phosphorothioate oligonucleotide. The process has the following steps: (a) providing an amount of a blocked nucleotide; (b) deblocking the blocked nucleotide to form an unblocked nucleotide; (c) activating the deblocked nucleotide; (d) coupling the deblocked nucleotide with a phosphoramidite to form a phosphite oligomer; (e) capping any uncoupled deblocked nucleotide via reaction with an amount of acetic anhydride and an amount of N-methylimidazole that is substantially free of 1,3,5-trimethylhexahydro-1,3,5-triazine; (f) oxidizing the phosphite oligomer to form the oligonucleotide or sulfurizing the phosphite oligomer to form a phosphorothioate oligonucleotide; and (g) optionally repeating steps (b) through (f). There is also a process for capping a nucleotide.

Abstract: A system (1) for the control of at least one unmanned aerial vehicle (2) from at least one aircraft (3), preferably a helicopter. The system (1) comprises at least one computer system (4), at least one satellite communication system (5) online with a satellite (6) and at least one radio set (7) for bidirectional data transfer between the aircraft (3) and the at least one unmanned aerial vehicle (2).

Abstract: According to the present invention, there is provided a process for making an oligonucleotide or a phosphorothioate oligonucleotide. The process has the following steps: (a) providing an amount of a blocked nucleotide; (b) deblocking the blocked nucleotide to form an unblocked nucleotide; (c) activating the deblocked nucleotide; (d) coupling the deblocked nucleotide with a phosphoramidite to form a phosphite oligomer; (e) capping any uncoupled deblocked nucleotide via reaction with an amount of acetic anhydride and an amount of N-methylimidazole that is substantially free of 1,3,5-trimethylhexahydro-1,3,5-triazine; (f) oxidizing the phosphite oligomer to form the oligonucleotide or sulfurizing the phosphite oligomer to form a phosphorothioate oligonucleotide; and (g) optionally repeating steps (b) through (f). There is also a process for capping a nucleotide.