Abstract: In one example embodiment, a system for stimulating tissue with the application of temperature-regulated fluid and negative pressure comprises a negative-pressure source adapted to provide negative pressure to a dressing. The system may further comprise a thermoelectric module thermally coupled to a first heat exchange chamber and a second heat exchange chamber and adapted to transfer heat between said heat exchange chambers. The thermoelectric module can be further adapted to maintain a temperature-regulated fluid fluidly coupled to the first heat exchange chamber and the dressing within a predetermined temperature range by adding heat to and extracting heat from the temperature-regulated fluid as it passes through the first heat exchange chamber.

Abstract: A device for generating skin grafts is disclosed, which includes a harvester having a distal end for placement on or in proximity of a portion of a subject's skin, where the harvester includes an orifice plate coupled to the distal end and having one or more openings. The device further includes a lid that is configured for attachment to the harvester to form a chamber and is configured for coupling to a vacuum source for generating a negative pressure in said chamber, thereby raising at least one blister through said at least one opening of the orifice plate. A skin-stretching mechanism is coupled to the harvester for stretching said skin portion so as to facilitate formation of said at least one blister. The harvester further includes a cutter integrated therein for cutting the blister. In some embodiments, the skin-stretching mechanism is coupled to the distal end of the harvester.

Abstract: A transfer dressing that can secure and/or prevent undesired movement of epidermal skin grafts transfer substrates is described. The transfer dressing can include a transfer substrate and one or more adhesive appendages that are configured to secure the transfer substrate to a recipient site (e.g., periwound area) in order to hold the transfer substrate in place and/or facilitate handling of the transfer substrate. The transfer substrate can be comprised of a fenestrated surface configured to facilitate harvesting and transfer of epidermal skin grafts.

Abstract: The present application relates to devices and methods for harvesting a skin graft(s) and introducing one or more nucleic acid, one or more amino acid sequences, or a combination thereof into one or more cells of the skin graft. The present invention provides transfecting the skin graft to modulate a cellular response that enhances epithelialization and/or pigmentation.