Abstract: In one example embodiment, a system for stimulating tissue with the application of temperature-regulated fluid and negative pressure comprises a negative-pressure source adapted to provide negative pressure to a dressing. The system may further comprise a thermoelectric module thermally coupled to a first heat exchange chamber and a second heat exchange chamber and adapted to transfer heat between said heat exchange chambers. The thermoelectric module can be further adapted to maintain a temperature-regulated fluid fluidly coupled to the first heat exchange chamber and the dressing within a predetermined temperature range by adding heat to and extracting heat from the temperature-regulated fluid as it passes through the first heat exchange chamber.

Abstract: The present application relates to devices and methods for harvesting a skin graft(s) and introducing one or more nucleic acid, one or more amino acid sequences, or a combination thereof into one or more cells of the skin graft. The present invention provides transfecting the skin graft to modulate a cellular response that enhances epithelialization and/or pigmentation.