Abstract: The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.

Abstract: The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.

Abstract: The present invention relates to a system (1) for infiltrating plant tissue with macromolecules or microorganisms. The system comprises an infiltration chamber (2) having at least one sealable hatch for receiving plant tissue, means (P1) for reducing pressure within the infiltration chamber; a first release valve (V1) in fluid communication with the infiltration chamber. The system further comprises a controller (C1) for operating coordinately the first release valve and the pressure reducing means such that the pressure in the infiltration chamber is (i) reduced from a start pressure to a target pressure that is less than or equal to 300 mbar in a first time period, (ii) maintained at the target pressure for a second time period, and (iii) increased from the target pressure to a final pressure of at least approximately 1 bar within a third time period of less than or equal to five (5) seconds. The present invention also relates to a method of infiltrating plant tissue with bacterium cells.

Abstract: The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.

Abstract: The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.