Abstract: An automatic forms handling application service provided on a global computer network, such as the Internet. A forms publisher submits a form and an associated report to the forms handling system. The form includes one or more data submission fields for accumulating data entries submitted into the form by visitors to the forms handling system. The report includes report elements for returning tabulated data entries accumulated using the form. The forms handling system automatically creates a database table corresponding to the form, and correlates each form submission field with a database storage element, such as a column in the database table, that accumulate data entries for its associated form submission field. The forms handling system also coordinates the database table with the associated report file, and periodically generates and delivers reports to the a specified location in accordance a service definition provided by the form publisher.

Abstract: An automatic forms handling application service provided on a global computer network, such as the Internet. A forms publisher submits a form and an associated report to the forms handling system. The form includes one or more data submission fields for accumulating data entries submitted into the form by visitors to the forms handling system. The report includes report elements for returning tabulated data entries accumulated using the form. The forms handling system automatically creates a database table corresponding to the form, and correlates each form submission field with a database storage element, such as a column in the database table, that accumulate data entries for its associated form submission field. The forms handling system also coordinates the database table with the associated report file, and periodically generates and delivers reports to the a specified location in accordance a service definition provided by the form publisher.

Abstract: A method for sequential cloning of chromosomal DNA of a target organism is disclosed. A first DNA segment homologous to the chromosomal DNA to be sequentially cloned is isolated. The first segment has a first restriction enzyme site on either side. A first vector product is formed by ligating the homologous segment into a suitably designed vector. The first vector product is circularly integrated into the target organism's chromosomal DNA. The resulting integrated chromosomal DNA segment includes the homologous DNA segment at either end of the integrated vector segment. The integrated chromosomal DNA is cleaved with a second restriction enzyme and ligated to form a vector-containing plasmid, which is replicated in a host organism. The replicated plasmid is then cleaved with the first restriction enzyme. Next, a DNA segment containing the vector and a segment of DNA homologous to a distal portion of the previously isolated DNA segment is isolated.

Abstract: A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme.

Abstract: Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252.

Abstract: Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.