Abstract: Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R1, R2, R3, R10, n and l are as defined in the specification.

Abstract: Provided herein are a primer set capable of amplifying target sequence(s) of nine respiratory disease-associated bacterial species, a probe set specifically hybridizing with the target sequence(s), a microarray comprising the probe set, and a method of detecting one or more of the nine respiratory disease-associated bacterial species using the probe set.

Abstract: Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R1, R2, R3, R10, n and l are as defined in the specification.

Abstract: Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.

Abstract: A method of increasing the specificity of nucleic acid hybridization, comprising hybridizing nucleic acid in a solution containing a zwitterionic compound selected from the group consisting of 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), 3-(cyclohexylamino)-2-hydroxy-1-propane sulfonate (CAPSO), and 2-(cyclohexylamino)ethane sulfonate (CHES) is provided. The method allows a reduction in the yield of non-specific amplification products in multiplex PCRs while maintaining the yield of target amplification products.

Abstract: Provided is a method of identifying a target cell using a virus. The method includes infecting the target cell with the virus by contacting the virus to the target cell and culturing the target cell to propagate the virus; adding a chromogenic substrate to the resultant cell culture to induce enzyme reaction converting the chromogenic substrate to a chromogenic product; and measuring an optical signal emitted from the chromogenic product, wherein the virus contains in its genome a gene encoding an enzyme capable of converting the chromogenic substrate to the chromogenic product and a gene encoding a ligand allowing the virus to specifically bind with a receptor of the target cell to infect the target cell with the virus.

Abstract: Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R1, R2, R3, R10, n and I are as defined in the specification.

Abstract: A microarray substrate comprising a functionalized (poly)ethyleneglycol compound coated on a solid support having a polyanhydride surface is disclosed. Methods of manufacturing and using the substrate are also disclosed.

Abstract: Provided are a nucleic acid purification apparatus including a chamber that includes an inlet and an outlet, at least two electrodes that defines the chamber and form an electric field, a photovoltaic device that applies a bias to the electrodes, a microfluidic apparatus including the same, and a method of purifying a nucleic acid using the nucleic acid purification apparatus. The nucleic acid purification apparatus includes the photovoltaic device and the conductive transparent electrodes, thus operating independently, and a PCR buffer can be used as an eluate for the nucleic acid. As a result, the nucleic acid purification apparatus can be integrated, and by using the apparatus, a fast and simplified purification can be obtained. The microfludic apparatus including the nucleic acid purification apparatus can operate independently, and perform the purification of the nucleic acid and a PCR at the same time. As a result, the microfluidic apparatus can be miniaturized and automated.

Abstract: Provided is a method of purifying nucleic acids using a metal-ligand complex, the method comprising: immobilizing the metal-ligand complex on a solid support; bringing a sample containing the nucleic acid into contact with the immobilized complex on the solid support to bind the nucleic acid to the complex; and adding a solution containing a chelate capable of removing the metal to elute the nucleic acid bound to the complex. The nucleic acids can be efficiently purified using a metal-ligand complex interacting with a base of the nucleic acid instead of interacting with a phosphoric acid of the nucleic acid to selectively bind the nucleic acid, and using a chelator capable of removing the metal to elute the nucleic acid.

Abstract: Provided is a method of identifying a target cell using a virus. The method includes infecting the target cell with the virus by contacting the virus to the target cell and culturing the target cell to propagate the virus; adding a chromogenic substrate to the resultant cell culture to induce enzyme reaction converting the chromogenic substrate to a chromogenic product; and measuring an optical signal emitted from the chromogenic product, wherein the virus contains in its genome a gene encoding an enzyme capable of converting the chromogenic substrate to the chromogenic product and a gene encoding a ligand allowing the virus to specifically bind with a receptor of the target cell to infect the target cell with the virus.

Abstract: Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.

Abstract: Provided is a microfluidic device including at least one inlet, at least one outlet, and a microchannel connecting the inlet and the outlet. The microfluidic device includes two or more electromagnets disposed on sidewalls of the microchannel and oriented in a predetermined direction with respect to the direction in which the microchannel extends.

Abstract: A cell lysis method in which an ionic additive and microwaves are used is provided. The cell lysis method includes: preparing a sample containing cells to be lysed; adding an ionic additive to the sample; and lysing cells in the sample by radiating microwaves onto the sample in which the ionic additive is added using a common microwave oven for a sufficient time to allow cell lysis to occur to a desired extent. According to the cell lysis method, even when a general microwave oven is used instead of expensive microwave equipment, regardless of the size of the sample, cell lysis can be rapidly performed by rapidly raising the temperature of the sample and a subsequent PCR process is not prohibited.

Abstract: Provided are an exothermic material, and a method and device for lysing cells using the exothermic material. The exothermic material is a solid material containing CaO, 5-7% by weight of MgCl2 or MgSO4, and 5-7% by weight of NaOH, with respect to the total weight of CaO, and generates heat upon the addition of sulfuric acid.