Abstract: Provided in one example is a system that includes one or more processors to receive a sequence data structure; apply at least one first metric of a plurality of metrics to the sequence data structure to generate at least one first metric score; determine that the at least one first metric score satisfies a first condition; apply, responsive to the at least one first metric score satisfying the first condition, at least one second metric of the plurality of metrics to the sequence data structure to generate at least one second metric score; and output an indication of the at least one first metric score and the at least one second metric score.

Abstract: Provided herein is a method of making, comprising transporting reagents to a reactor in a microfluidic path device, wherein the reagents include a synthetic gene of interest, a polymerase, a buffer, a first primer having a first region specific to the synthetic gene of interest, and a second primer, wherein the second primer comprises a poly-T sequence of ?150 base pairs (bp) or a poly-A sequence of ?150 bp and a second region specific to the synthetic gene of interest; controlling a temperature of the first reactor to perform a polymerase chain reaction within the microfluidic path device to amplify the synthetic gene of interest using the first primer and the second primer to form a synthetic product including the poly-A sequence of ?150 bp; and transporting the synthetic product out of the first reactor, wherein the synthetic product comprises a synthetic DNA template for in vitro transcription.

Abstract: Methods and apparatuses for making and using therapeutics, including in particular mRNA therapeutics, that separate double-stranded RNA from single-stranded RNA as part of a continuous flow. These methods and apparatuses may include formulation of an RNA therapeutic using a permeable insert integrated into a microfluidic path device. In particular, these methods and apparatuses may include formulation of an RNA therapeutic by removing dsRNA from a solution of RNA by within a microfluidic path device including a cellulose material.