Abstract: The present invention provides a method for modulating (e.g. decreasing) the nicotine content of a plant (e.g. a tobacco plant) or part thereof, or tobacco plant cell, the method comprising modifying said plant or cell providing at least one mutation in a Nic3 locus. The present invention provides a method for modulating (e.g. decreasing) the nicotine content of a plant (e.g. a tobacco plant) or part thereof, or tobacco plant cell, the method comprising modifying said plant or cell to modulate the expression or activity of at least one Nic3 gene. The present invention also provides for the use of the Nic3 locus for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or delivery systems obtainable in accordance with the invention.

Abstract: The present invention relates to a method of modulating the alkaloid content of a plant or a part thereof, the method comprising modifying said plant by modulating the activity or expression of at least one gene encoding an RNA binding protein. The present invention also relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) precursor in tobacco, the method comprising modulating the activity or expression of at least one gene encoding an RNA binding protein.

Abstract: The present invention relates to a method of modulating the alkaloid content of a plant or a part thereof, the method comprising modifying said plant by modulating the activity or expression of at least one gene encoding a BTB/POZ NPH3 domain-containing protein. The present invention also relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) precursor in tobacco, the method comprising modulating the activity or expression of at least one gene encoding a BTB/POZ NPH3 domain-containing protein.

Abstract: The present invention relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) precursor in tobacco, the method comprising modulating the activity or expression of at least one gene encoding a Walls are thin 1-related (WAT1-related) protein.

Abstract: The present invention provides a method for modulating (e.g. decreasing) the alkaloid content of a plant (e.g. a tobacco plant), the method comprising modifying said plant by modulating the activity or expression of at least one gene encoding a SOUL haem-binding protein. The present invention also provides for the use of at least one gene encoding a SOUL haem-binding protein for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.

Abstract: The present invention provides a method for modulating (e.g. decreasing) the alkaloid content of a plant (e.g. a tobacco plant), the method comprising modifying said plant by modulating (e.g. decreasing) the expression of at least one armadillo repeat protein. The present invention also provides for the use of an armadillo repeat protein for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.

Abstract: The present invention provides a method for modulating the alkaloid content of a plant (e.g. a tobacco plant), the method comprising modifying said plant by modulating the activity or expression of at least one protein kinase. The present invention also provides for the use of at least protein kinase gene for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.

Abstract: The present invention relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) or a precursor of a TSNA in tobacco comprising expressing a deregulated cation efflux protein in a tobacco plant, or plant part thereof, or plant cell.