Abstract: In one example embodiment, a plurality of objects selected by a user on a user interface for a collaboration tool are identified. The plurality of objects include at least two different types of objects. One or more collaboration actions to perform are determined by a computing device based on the types and contextual information for the plurality of objects. The one or more collaboration actions are presented on the user interface. A presented collaboration action is initiated from the user interface.

Abstract: Scoring functions for predicting response of a dMMR and/or MSI-H colorectal tumor to a PD-1 axis-directed therapy are disclosed, as well as methods and systems for evaluating tissue samples for the presence of feature metrics useful in computing such scoring functions. The scoring functions integrate one or more spatial relationships between cell types into a numerical indication of the likelihood that the tumor will respond to the PD-1 axis-directed therapy. Based on the output of the scoring function, a subject may then be selected to receive a PD-1 axis-directed therapy (if the scoring function indicates a sufficient likelihood of positive response) or an alternative therapy (if the scoring function indicates an insufficient likelihood of positive response).

Abstract: Provided is a polymer comprising a hydrolysable polymer backbone, the polymer backbone comprising: (i) monomer units comprising a hydrophobic side chain; (ii) monomer units comprising a side chain comprising a polyamine group and a polyalkylene oxide group; and, optionally, (iii) monomer units comprising a side chain comprising a polyamine group without a polyalkylene oxide group, as well as a method of preparing said polymer, and a method of delivering a nucleic acid and/or polypeptide to a cell using the polymer.

Abstract: Pruning shear heads are provided. A pruning shear head includes a base portion; a blade movable relative to the base portion between an open position and a closed position; and a branch hook comprising a contoured interior face, the branch hook being movable relative to the base portion between an open position and a stowed position.

Abstract: A bra includes an adjustable body strap that has a rear portion with first and second coupling features disposed on a single side thereof, and first and second support straps that are respectively coupled at one end to the rear portion of the body strap. Each of the first and second support straps includes a fastening material, first and second fabric materials, and a flexibly resilient material. The first fabric, the flexibly resilient material, the second fabric, and the fastening material are integrally formed to define a laminated support strap of each of the first and second support straps.

Abstract: In one example embodiment, a plurality of objects selected by a user on a user interface for a collaboration tool are identified. The plurality of objects include at least two different types of objects. One or more collaboration actions to perform are determined by a computing device based on the types and contextual information for the plurality of objects. The one or more collaboration actions are presented on the user interface. A presented collaboration action is initiated from the user interface.

Abstract: The present disclosure relates to immunomodulatory compositions that selectively activate TLR7, comprising an RNA ligand and a pharmaceutically acceptable carrier, as well as to in vitro and therapeutic uses of such immunomodulatory compositions.

Abstract: Provided are methods and compositions for the heterologous expression of a payload (e.g., DNA, RNA, protein) of interest in a target cell (e.g., cancer cell). In some cases payload delivery results in expression (e.g., by a cancer cell in vivo) of a secreted immune signal such as a cytokine, a plasma membrane-tethered affinity marker (thus resulting in an induced immune response), or a cytotoxic protein such as an apoptosis inducer (e.g., by a cancer cell in vivo). Payloads are delivered with a delivery vehicle and in some cases the delivery vehicle is a nanoparticle. In some cases a subject nanoparticle includes a targeting ligand for targeted delivery to a specific cell type/tissue type (e.g., a cancerous tissue/cell). In some embodiments, payload delivery is “personalized” in the sense that the delivery vehicle and/or payload can be designed based on patient-specific information.

Abstract: A flood column for charged particle flooding of a sample, the flood column comprising a charged particle source configured to emit a charged particle beam along a beam path; a source lens arranged down-beam of the charged particle source; a condenser lens arranged down-beam of the source lens; and an aperture body arranged down-beam of the condenser lens, wherein the aperture body is for passing a portion of the charged particle beam; and wherein the source lens is controllable so as to variably set the beam angle of the charged particle beam down-beam of the source lens.

Abstract: A bra includes first and second cups that are laterally spaced-apart. The first and second cups each have upper edges. First and second support straps are selectively coupled to the upper edges of the first and second cups. The first and second support straps each include a first end that has a first coupling feature and a body portion that has a second coupling feature. The second coupling feature is a reciprocal coupling feature to the first coupling feature. Each of the first and second support straps also includes a second end that has a retention feature, first and second fabrics, a pliable material, and a fastening material. The fastening material includes the first and second coupling features. The first fabric, the pliable material, the second fabric, and the fastening material are integrally formed to define a laminated support strap of each of the first and second support straps.

Abstract: A bra includes an adjustable body strap that has a rear portion with first and second coupling features disposed on a single side thereof, and first and second support straps that are respectively coupled at one end to the rear portion of the body strap. Each of the first and second support straps includes a fastening material, first and second fabric materials, and a flexibly resilient material. The first fabric, the flexibly resilient material, the second fabric, and the fastening material are integrally formed to define a laminated support strap of each of the first and second support straps.

Abstract: A brassiere and garment system includes a garment having a tubular body portion with an upper edge, wherein a rear portion of the upper edge downwardly drapes between first and second sides of the tubular body portion. First and second fasteners each include an anchor portion defining a mounting aperture and a coupling portion defining a receiving aperture disposed above the mounting aperture. The brassiere includes first and second support straps having free ends that are configured to be received through the receiving apertures of the first and second fasteners, respectively, such that the garment is suspended from the adjustable first and second straps of the brassiere in use.

Abstract: A magnetic fastener includes a female component and a male component. The female component includes a base, a hooded portion spaced from the base and formed opposite thereto, and a peripheral wall connecting the base to the hooded portion. A pocket is formed between the base and the hooded portion. The hooded portion includes a slot formed therein. A portion of the female component is magnetically attractive. The male component includes an insertion portion and a shaft extending from the insertion portion. A portion of the male component is formed by a magnet. The male component is configured to be coupled to the female component when the magnet is magnetically coupled to the portion of the female component that is magnetically attractive, the insertion portion is received within the pocket, and the shaft is received within the slot.

Abstract: A magnetic fastener includes a female component and a male component. The female component includes a base, a hooded portion spaced from the base and formed opposite thereto, and a peripheral wall connecting the base to the hooded portion. A pocket is formed between the base and the hooded portion. The hooded portion includes a slot formed therein. A portion of the female component is magnetically attractive. The male component includes an insertion portion and a shaft extending from the insertion portion. A portion of the male component is formed by a magnet. The male component is configured to be coupled to the female component when the magnet is magnetically coupled to the portion of the female component that is magnetically attractive, the insertion portion is received within the pocket, and the shaft is received within the slot.

Abstract: Provided are methods and compositions for genome editing using sticky ends. Subject methods include (a) generating a staggered cut at each of two locations in genomic DNA of a target cell, thus generating two genomic staggered ends; and (b) providing/introducing a linear double stranded donor DNA that has staggered ends (i.e., sticky ends) that match/correspond to the sticky ends of the genomic DNA such that the sticky ends of the donor DNA hybridize with the sticky ends of the genomic DNA and the donor DNA is inserted into the genome. In some cases, the staggered cuts are generated by introducing into a target cell one or more sequence specific nucleases (or one or more nucleic acids encoding the one or more sequence specific nucleases).

Abstract: The present disclosure provides immunotherapeutic compositions and methods for enhancing an immune response and for treating cancer or inflammatory conditions mediated by autoreactive B cells in a subject. In some aspects, multispecific antigen-binding constructs are provided that recognize at least one tumor antigen or B-lineage cell antigen and NKp30 and/or another activating NK receptor. In some aspects, multispecific antigen-binding constructs are provided that recognize at least two tumor antigens or two antigens expressed by B-lineage cells, NKp30, and another activating NK receptor. The multispecific antigen-binding constructs and methods disclosed herein can be used for the treatment of cancer, even a cancer characterized by a CD16 deficient microenvironment and/or characterized by target cells (e.g., cancer cells) having a low level of expression of the tumor antigen.

Abstract: The present disclosure provides immunotherapeutic compositions and methods for enhancing an immune response and for treating cancer or inflammatory conditions mediated by autoreactive B cells in a subject. In some aspects, multispecific antigen-binding constructs are provided that recognize at least one tumor antigen or B-lineage cell antigen and NKp30 and/or another activating NK receptor. In some aspects, multispecific antigen-binding constructs are provided that recognize at least two tumor antigens or two antigens expressed by B-lineage cells, NKp30, and another activating NK receptor. The multispecific antigen-binding constructs and methods disclosed herein can be used for the treatment of cancer, even a cancer characterized by a CD16 deficient microenvironment and/or characterized by target cells (e.g., cancer cells) having a low level of expression of the tumor antigen.

Abstract: A magnetic fastener including a female component and a male component, each of the female component and the male component coupled to a magnet. When assembled, the magnet of the male component and the magnet of the female component are magnetically engaged and cooperate to militate against undesired disengagement of the magnetic fastener.

Abstract: Provided are minimal hammerhead ribozymes and catalytic strands thereof. Aspects of the present disclosure include a catalytic strand of a minimal hammerhead ribozyme, the catalytic strand including a catalytic core region, a stem I-forming region, a stem II region, and a stem III-forming region. The catalytic strand hybridizes to a target strand via the stem I-forming region and the stem III-forming region. A nucleotide (e.g., an adenine, cytosine, or a natural or non-natural nucleotide base having hydrogen bond donor and acceptor functionalities at positions analogous to those of adenine or cytosine) present in a stem II loop base pairs with a nucleotide (e.g., uracil or cytosine) at position 1.7 of the target strand. Also provided are compositions that include the catalytic strands, and methods of using the catalytic strands, e.g., in a variety of different applications, as well as kits that find use in practicing embodiments of the methods.

Abstract: An endoluminal prosthesis for placement within a body vessel may include a tubular support structure including a proximal end segment, a distal end segment, an intermediate segment positioned between the proximal end segment and the distal end segment, a lumen extending longitudinally within the support structure, a luminal surface, and an abluminal surface opposite the luminal surface. The prosthesis may include a first layer of nonwoven electrospun fibers positioned on the luminal surface of the support structure. The prosthesis may include a second layer of nonwoven electrospun fibers positioned on the abluminal surface of the support structure. At least one of the proximal end segment or the distal end segment of the support structure may be encapsulated within a covering including the first layer of nonwoven electrospun fibers and the second layer of nonwoven electrospun fibers. The intermediate segment of the support structure may be unencapsulated within the covering.