Abstract: The present disclosure provides iPSC-derived dopaminergic neurons comprised disease-associated mutations. Further provided herein are methods for using the iPSC-derived dopaminergic neurons for the study of neuroinflammation, such as to identify novel targets, biomarkers, and therapeutic agents for the diagnosis, prognosis, and treatment of neurodegenerative diseases. Further provided herein are assays for studying neuroinflammation using the present cell culture models.

Abstract: Provided herein are methods of producing cell lines with stable expression of a transgene by removal of CpG motifs. In further methods, there are provided methods for cell lines with stable expression of a transgene by driving expression by novel promoters or by tagging endogenous genes.

Abstract: The present disclosure provides methods of producing progenitor cells from induced pluripotent stem cells, wherein the progenitor cells comprise endothelial cells, pericytes, brain microvascular endothelial cells (BMECs), mesenchymal stem cells (MSCs), hematopoietic precursor cells (HPCs), microglia or neural precursor cells (NPCs).

Abstract: Described herein is a method for producing a sealant. The method includes mixing a first material with a second material at a manufacturing site to produce the sealant. The method also includes applying x-ray energy to the sealant at the manufacturing site. The method includes measuring an amount of fluorescence emitted from the sealant in response to applying the x-ray energy. The method also includes calculating a mix ratio of the first and second materials of the sealant based on the amount of fluorescence. The method includes determining whether the mix ratio is within a predetermined mix ratio range.

Abstract: Described herein is a method for producing a sealant. The method includes mixing a first material with a second material at a manufacturing site to produce the sealant. The method also includes applying x-ray energy to the sealant at the manufacturing site. The method includes measuring an amount of fluorescence emitted from the sealant in response to applying the x-ray energy. The method also includes calculating a mix ratio of the first and second materials of the sealant based on the amount of fluorescence. The method includes determining whether the mix ratio is within a predetermined mix ratio range.

Abstract: Described herein is a method for producing a sealant. The method includes mixing a first material with a second material at a manufacturing site to produce the sealant. The method also includes applying x-ray energy to the sealant at the manufacturing site. The method includes measuring an amount of fluorescence emitted from the sealant in response to applying the x-ray energy. The method also includes calculating a mix ratio of the first and second materials of the sealant based on the amount of fluorescence. The method includes determining whether the mix ratio is within a predetermined mix ratio range.