Abstract: Cell-culture platforms are described that can impart cyclic strain on a biologically-relevant culture substrate. Accordingly, systems and methods of the invention can provide a more accurate model of physiological strain and forces than earlier technologies. Systems of the invention enable high-throughput experimentation in standard culturing equipment, while utilizing a 3D physiologically-relevant environment.

Abstract: Functionalized molecular layers comprising a backbone of polymerized amphiphiles for placement on a surface of a scaffold, such as a hydrogel. Methods of preparing such molecular layers and hydrogels comprising the same are also provided, as are methods of use thereof.

Abstract: A tissue stretching system includes a motor, a first pillar, a second pillar, a force sensor, and an imaging device. The tissue stretching system may be provided as a 2D axial tissue stretcher that may monitor changes in mechanical properties over time. Tissues may be coated onto the first pillar and/or the second pillar. In a specific example, the first pillar and/or the second pillar may be a PDMS (Polydimethylsiloxane) structure configured to probe the mechanical properties in between a designed gap of the first pillar and the second pillar. The motor of the tissue stretching system includes a microcontroller. The motor of the tissue stretching system may have high resolution capabilities to provide the appropriate strain to the sample. The tissue stretching system then determines the actual amount of strain applied to the sample.

Abstract: Cell-culture platforms are described that can impart cyclic strain on a biologically-relevant culture substrate. Accordingly, systems and methods of the invention can provide a more accurate model of physiological strain and forces than earlier technologies. Systems of the invention enable high-throughput experimentation in standard culturing equipment, while utilizing a 3D physiologically-relevant environment.