Abstract: The present invention relates to a novel closed linear DNA vector, which is suitable for use in the production of lentiviral particles. Notably, the present invention relates to a new configuration of the vector including the transgene (often termed the “payload” vector), which enables a greater yield of infectious lentiviral particles, notably a greater yield of lentiviral particles carrying a trans-gene, to be prepared when compared to closed linear DNA vectors lacking this configuration. Further, the inventors have developed improvements in lentiviral production with closed linear DNA, through optimisation of vector input quantities and construct ratios. The invention furthermore relates to a method of generating infectious lentiviral particles using the construct, optionally in conjunction with improved production vectors and/or optimised methodology.

Abstract: A method of processing media output events, each having a corresponding duration, from a plurality of media content receivers within a media distribution system, comprises: a. dividing the output events into a plurality of segments, such that output events having similar properties are placed into the same one of said segments; b. within each said segment, identifying a long duration threshold based on the distribution of the durations of the output events within that segment; c. and applying the long duration thresholds of each said segment for control and/or analysis of the media distribution system.

Abstract: A method of processing media output events, each having a corresponding duration, from a plurality of media content receivers within a media distribution system, comprises: a. dividing the output events into a plurality of segments, such that output events having similar properties are placed into the same one of said segments; b. within each said segment, identifying a long duration threshold based on the distribution of the durations of the output events within that segment; c. and applying the long duration thresholds of each said segment for control and/or analysis of the media distribution system.

Abstract: Detergent compositions and detergent compositions dispersed in personal care products are provided herein. In one embodiment, a detergent composition is provided and comprises a sorbent carrier. The detergent composition also includes a sorbed component including an acid and a surfactant. The sorbed component is sorbed by the sorbent carrier.

Abstract: Detergent compositions and detergent compositions dispersed in personal care products are provided herein. In one embodiment, a detergent composition is provided and comprises a sorbent carrier. The detergent composition also includes a sorbed component including an acid and a surfactant. The sorbed component is sorbed by the sorbent carrier.

Abstract: A mobility assistance vest can be easily donned by placing the vest inside up under the dog, then pulling the right and left side sections together over the top line fastening the Velcro strips, and then pulling the chest section by the front shoulder straps through the front legs and securing the shoulder straps to the side sections on either side of the neck. The semi-detachable handle straps at the front legs are then crossed to the opposing side and attached with a snap to D-rings on the shoulder handles for the human handler to provide counter weight or upward support as they both descend the stairs side by side. Alternatively, the detachable handle straps at the front legs can be attached with the snap connector to the D-rings on the rear section of the vest to provide support for assisting the canine while ascending stairs, walking and standing up.