Abstract: A multiplexed polymerase chain reaction (PCR) DNA detection system and a method for DNA detection within the PCR system are provided. The PCR DNA detection system includes a color charge-coupled device (CCD) camera, fluorophore-quencher probes and an imaging chamber. The fluorophore-quencher probes are selected in response to fluorophores quenched by the fluorophore-quencher probes corresponding to three selected primary colors and peak channel responses of the CCD camera such that emission profiles of the fluorophores substantially match the three selected primary colors and peak emission profiles of the fluorophores correspond to the peak RGB channel responses of the CCD camera. A DNA sample and the fluorophore-quencher probes are located within the imaging chamber. The color CCD camera is focused on the imaging chamber for simultaneous detection of up to three targets from fluorescence of the DNA sample and the fluorophore-quencher probes.

Abstract: There is provided a microfluidic device comprising: a plurality of wells, each well having an inlet and an outlet, wherein the inlets are in fluid communication with one or more entry channels and the outlets are in fluid communication with one or more exit channels, wherein said outlet is connected to the exit channel via an outlet connecting channel and said inlet is connected to the entry channel via an inlet connecting channel wherein the dimension of the outlet connecting channel is configured such that the surface tension of a liquid comprised in the well prevents the release of the liquid through the outlet connecting channel. There is also provided a system, method and use of the device.

Abstract: A colorimetric-based DNA diagnostic system which includes a detector module, a processor and a memory is provided. The detector module is disposed to record an image of a DNA sample illuminated by a light source. The memory includes computer program code which along with the memory is configured, with the processor, to perform (a) sending a signal to adjust the temperature of the DNA sample to be within an approximate temperature range over which the color of the DNA sample changes, (b) sending a signal to the detector module to capture an image of the DNA sample at defined intervals within the approximate temperature range, (c) processing the captured images to extract color information, and (d) processing the extracted color information to objectively determine a melting temperature within the approximate temperature range at which the color of the DNA sample changes.

Abstract: There is provided a microfluidic device comprising: a plurality of wells, each well having an inlet and an outlet, wherein the inlets are in fluid communication with one or more entry channels and the outlets are in fluid communication with one or more exit channels, wherein said outlet is connected to the exit channel via an outlet connecting channel and said inlet is connected to the entry channel via an inlet connecting channel wherein the dimension of the outlet connecting channel is configured such that the surface tension of a liquid comprised in the well prevents the release of the liquid through the outlet connecting channel. There is also provided a system, method and use of the device.

Abstract: A multiplexed polymerase chain reaction (PCR) DNA detection system and a method for DNA detection within the PCR system are provided. The PCR DNA detection system includes a color charge-coupled device (CCD) camera, fluorophore-quencher probes and an imaging chamber. The fluorophore-quencher probes are selected in response to fluorophores quenched by the fluorophore-quencher probes corresponding to three selected primary colors and peak channel responses of the CCD camera such that emission profiles of the fluorophores substantially match the three selected primary colors and peak emission profiles of the fluorophores correspond to the peak RGB channel responses of the CCD camera. A DNA sample and the fluorophore-quencher probes are located within the imaging chamber. The color CCD camera is focused on the imaging chamber for simultaneous detection of up to three targets from fluorescence of the DNA sample and the fluorophore-quencher probes.

Abstract: There is provided a method of monitoring retinopathy in a subject. The method involves measuring autofluorescence of a retina in response to high intensity blue light and infra-red reflectance of the anterior region of an eye in response to high intensity infra-red light of the subject over a total time period to obtain an autofluorescence intensity profile and an infra-red reflectance intensity profile. The autofluorescence intensity profile and infra-red reflectance intensity profile are processed to obtain a pupillary light reflex measurement that is used to assess the retinopathy status of the retina.