Abstract: “BIO-PESTICIDE OIL FORMULATION” The present invention relates to a bio-pesticide formulation and a process for the preparation thereof. Particularly this invention relates to a formulation useful against foliar plant diseases, wherein said bio-pesticide formulation comprises of: I.) a homogeneous component I comprising: Eucalyptus leaves; Trichoderma harzianum; and karanj cake, II.) a component II comprising: an agro oil in 50 to 80% of component II; water in 10 to 30% of component II; and one or more emulsifiers selected from the group consisting of glycerine, Tween 20 and propylene glycol in 02 to 30% of component II, III.) a carbon source in a percentage of 2 to 10%, and IV.) optionally a structural agent in a percentage of 2 to 10%.