Abstract: The present invention relates to a fusion protein for use in ubiquitin-independent protein degradation. The invention also relates to methods for the use of the fusion protein in targeted protein degradation, modulating physiological responses and therapy.

Abstract: The complete genomic sequence of maize fine streak virus (MFSV), a negative-strand RNA virus that infects plants and insects, is disclosed. The inventors have characterized the MFSV genome and identified the leader and trailer sequences, seven open reading frames as well as the functions of some of the encoded proteins, and the gene junction sequences and their functions. Using various functionally important components of the MFSV genome, the inventors have demonstrated that a reverse genetics system of using cloned cDNA to produce infectious viruses can be developed for plant negative-strand RNA viruses. Methods of using the reverse genetic system to produce wild-type or recombinant plant negative-strand RNA viruses, to express genes of interest, and to screen for agents that may affect the production and function of the viruses are disclosed. Further disclosed are various nucleic acid constructs, vectors, genetically engineered cells, and kits useful in the reverse genetics system.