Patents by Inventor Satori Sone

Satori Sone has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6472196
    Abstract: In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in Rhodosporidium toruloides, and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3′-terminus of the promoter region and the 5′-terminus of the terminator region. Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established. Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.
    Type: Grant
    Filed: March 15, 1994
    Date of Patent: October 29, 2002
    Assignee: Mitsu Chemicals, Inc.
    Inventors: Nobuhiro Fukuhara, Setsuo Yoshino, Kaoru Yamamoto, Tomoyuki Se, Satori Sone, Yoshiyuki Nakajima, Maki Suzuki, Nobuyoshi Makiguchi
  • Patent number: 5322786
    Abstract: Escherichia coil carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40.degree. C. or over so that the expression of said desired foreign gene was suppressed. This E. coil (i.e., transformant) was cultured at 40.degree. C. or over in a first process to suppress the expression of the foreign gene and to support sufficient cell growth and thereafter below 40.degree. C. in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.
    Type: Grant
    Filed: November 27, 1991
    Date of Patent: June 21, 1994
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Nobuhiro Fukuhara, Setsuo Yoshino, Kaoru Yamamoto, Satori Sone, Maki Suzuki, Yoshiyuki Nakajima
  • Patent number: 5139935
    Abstract: Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process so as to supress the suppression of the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.
    Type: Grant
    Filed: February 25, 1991
    Date of Patent: August 18, 1992
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Nobuhiro Fukuhara, Setsuo Yoshino, Satori Sone, Yoshiyuki Nakajima, Nobuyoshi Makiguchi
  • Patent number: 5043277
    Abstract: Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40.degree. C. or over so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured at 40.degree. C. or over in a first process to suppress the expression of the foreign gene and to support sufficient cell growth and thereafter below 40.degree. C. in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.
    Type: Grant
    Filed: February 17, 1988
    Date of Patent: August 27, 1991
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Nobuhiro Fukuhara, Setsuo Yoshino, Kaoru Yamamoto, Satori Sone, Maki Suzuki, Yoshiyuki Nakajima
  • Patent number: 4946790
    Abstract: A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P.sub.L promoter of the lambda phage, the tac promoter and the P.sub.L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.
    Type: Grant
    Filed: February 1, 1988
    Date of Patent: August 7, 1990
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Nobuhiro Fukuhara, Setsuo Yoshino, Satori Sone, Yoshiyuki Nakajima, Nobuyoshi Makiguchi