Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.

Abstract: The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly. The method of the present invention for binding a protein to a carrier comprises: preparing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Abstract: The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.

Abstract: The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly. The method of the present invention for binding a protein to a carrier comprises: preparing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds.

Abstract: The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

Abstract: The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

Abstract: The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

Abstract: The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

Abstract: As means for giving cold-stability to plants, a novel polypeptide having cold-stable pyruvate, orthophosphate dikinase activity, a cloned DNA encoding the same, and a recombinant vector containing the DNA are disclosed. The polypeptide according to the present invention has cold-stable pyruvate, orthophosphate dikinase activity, which has an amino acid sequence that is the same as the amino acid sequence of 1/6 region of the entire region from the C-terminal of the following polypeptide (1) or (2), except that at least one amino acid residues of said 1/6 region are substituted with other amino acid residues:(1) a pyruvate, orthophosphate dikinase having an amino acid sequence shown in SEQ ID NOS. 1-10 in Sequence Listing; and(2) a polypeptide having an amino acid sequence which has a homology of not less than 50% with the amino acid sequence mentioned in (1), said polypeptide having cold-stable pyruvate, orthophosphate dikinase activity.