Abstract: The purpose of the present invention is to provide: a novel method for detecting a target nucleic acid; and a kit for use in the method. In the detection method according to the present invention, a fluorophore-labeled primer/probe and a quencher-labeled probe, which have complementarity to each other, are so designed as to have different melting temperatures (Tm) from each other so that the fluorophore-labeled primer can anneal preferentially to the target nucleic acid. The detection method is so designed that the fluorophore-labeled primer/probe that is not bound to the target nucleic acid is bound to the quenching probe so as to emit no fluorescence. The method enables the detection of the target nucleic acid in a simpler manner, at lower cost, and without requiring the use of any technique or device.

Abstract: The purpose of the present invention is to provide: a novel method for detecting a target nucleic acid; and a kit for use in the method. In the detection method according to the present invention, a fluorophore-labeled primer/probe and a quencher-labeled probe, which have complementarity to each other, are so designed as to have different melting temperatures (Tm) from each other so that the fluorophore-labeled primer can anneal preferentially to the target nucleic acid. The detection method is so designed that the fluorophore-labeled primer/probe that is not bound to the target nucleic acid is bound to the quenching probe so as to emit no fluorescence. The method enables the detection of the target nucleic acid in a simpler manner, at lower cost, and without requiring the use of any technique or device.

Abstract: A crankshaft support structure of an internal combustion engine, comprising a crankshaft having journal portions a saddle-shaped bearing caps respectively mounted on an undersurfaces of the journal support bases formed in a lower portion of the cylinder block in such a manner as to straddle the journal portions being accommodated in the bearing portions, whereby the journal portions are rotatably supported wherein, of the undersurface of each of the journal support bases, an undersurface portion overlapping with an edge portion of each of end faces on both sides of each of the bearing caps is formed to be a thick portion thicker than other portions, and a mount surface to be positioned on the undersurface overlapping with the thick portion of each of the bearing caps is formed along the thick portion.

Abstract: A crankshaft support structure of an internal combustion engine, comprising a crankshaft having journal portions a saddle-shaped bearing caps respectively mounted on an undersurfaces of the journal support bases formed in a lower portion of the cylinder block in such a manner as to straddle the journal portions being accommodated in the bearing portions, whereby the journal portions are rotatably supported wherein, of the undersurface of each of the journal support bases, an undersurface portion overlapping with an edge portion of each of end faces on both sides of each of the bearing caps is formed to be a thick portion thicker than other portions, and a mount surface to be positioned on the undersurface overlapping with the thick portion of each of the bearing caps is formed along the thick portion.