Patents by Inventor Satoshi Tsuneda
Satoshi Tsuneda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9587271Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: GrantFiled: October 31, 2012Date of Patent: March 7, 2017Assignee: NIPPON STEEL & SUMIKIN ECO-TECH CORPORATIONInventors: Kazunori Nakamura, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20150368712Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.Type: ApplicationFiled: September 8, 2015Publication date: December 24, 2015Inventors: Yuji SEKIGUCHI, Naohiro NODA, Ryo MIYATA, Kazunori NAKAMURA, Shinya KURATA, Satoshi TSUNEDA, Hidenori TANI
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Publication number: 20140178876Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.Type: ApplicationFiled: December 2, 2013Publication date: June 26, 2014Applicant: Nippon Steel & Sumikin Eco-Tech CorporationInventors: Yuji SEKIGUCHI, Naohiro NODA, Ryo MIYATA, Kazunori NAKAMURA, Shinya KURATA, Satoshi TSUNEDA, Hidenori TANI
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Publication number: 20130065237Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: October 31, 2012Publication date: March 14, 2013Inventors: Kazunori NAKAMURA, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20110224417Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: April 19, 2011Publication date: September 15, 2011Applicants: KANKYO ENGINEERING CO., LTD., NATIONAL INSTITUTE OF ADV. INDUSTRIAL SCI. & TECH.Inventors: KAZUNORI NAKAMURA, TAKAHIRO KANAGAWA, NAOHIRO NODA, SATOSHI TSUNEDA, HIDENORI TANI, SHINYA KURATA
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Publication number: 20110212442Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.Type: ApplicationFiled: July 30, 2009Publication date: September 1, 2011Applicant: Nippon Steel Kankyo Engineering Co., Ltd.Inventors: Yuji Sekiguchi, Naohiro Noda, Ryo Miyata, Kazunori Nakamura, Shinya Kurata, Satoshi Tsuneda, Hidenori Tani
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Patent number: 7960171Abstract: The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium oxidizing bacteria with ammonium and nitrite as substrates. Equipment for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank, novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device which feeds ammonium at a given concentration into the cultivation tank, a nitrite feed device which feeds nitrite at a given concentration into the cultivation tank, and a control device which controls a feed rate Y of the substrate.Type: GrantFiled: May 6, 2008Date of Patent: June 14, 2011Assignee: Hitachi Plant Technologies, Ltd.Inventors: Kazuichi Isaka, Tatsuo Sumino, Satoshi Tsuneda
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Patent number: 7951604Abstract: To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: GrantFiled: August 13, 2009Date of Patent: May 31, 2011Assignees: Kankyo Engineering Co., Ltd., National Institute of Advanced Industrial Science and TechnologyInventors: Kazunori Nakamura, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20100015719Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: August 13, 2009Publication date: January 21, 2010Applicants: KANKYO ENGINEERING Co., Ltd., National Institute of Adv. Industrial Sci. & Tech.Inventors: Kazunori NAKAMURA, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20080280352Abstract: The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium oxidizing bacteria with ammonium and nitrite as substrates. Equipment for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank, novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device which feeds ammonium at a given concentration into the cultivation tank, a nitrite feed device which feeds nitrite at a given concentration into the cultivation tank, and a control device which controls a feed rate Y of the substrate.Type: ApplicationFiled: May 6, 2008Publication date: November 13, 2008Applicant: Hitachi Plant Technologies, Ltd.Inventors: Kazuichi Isaka, Tatsuo Sumino, Satoshi Tsuneda
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Patent number: 7442539Abstract: The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium oxidizing bacteria with ammonium and nitrite as substrates. Equipment for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank, novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device which feeds ammonium at a given concentration into the cultivation tank, a nitrite feed device which feeds nitrite at a given concentration into the cultivation tank, and a control device which controls a feed rate Y of the substrate.Type: GrantFiled: March 13, 2006Date of Patent: October 28, 2008Assignee: Hitachi Plant Technologies, Ltd.Inventors: Kazuichi Isaka, Tatsuo Sumino, Satoshi Tsuneda
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Publication number: 20070128608Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: December 20, 2004Publication date: June 7, 2007Applicants: Kankyo Engineering Co., Ltd., National Institute of Adv. Industrial Sci. & Tech.Inventors: Kazunori Nakamura, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20060205055Abstract: The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium oxidizing bacteria with ammonium and nitrite as substrates. Equipment for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank, novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device which feeds ammonium at a given concentration into the cultivation tank, a nitrite feed device which feeds nitrite at a given concentration into the cultivation tank, and a control device which controls a feed rate Y of the substrate.Type: ApplicationFiled: March 13, 2006Publication date: September 14, 2006Applicant: Hitachi Plant Engineering & Construction Co., Ltd.Inventors: Kazuichi Isaka, Tatsuo Sumino, Satoshi Tsuneda
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Publication number: 20060054099Abstract: An aquarium-cleaning device, particularly an aquarium-cleaning device in which spent grain charcoal is used as formed charcoal. The formed charcoal provided by drying, forming, and carbonizing organic substances produced in food industries is used as a microorganism carrier. Such problems with a conventional device that the structure of the aquarium-cleaning device is complicated, the washing operation thereof is troublesome, and a filter medium must be frequently replaced can be solved by using the spent grain charcoal performing higher water-quality purification than a conventional activated charcoal as an aquarium-cleaning material.Type: ApplicationFiled: November 28, 2003Publication date: March 16, 2006Applicant: ASAHI BREWERIES, LTDInventors: Hiroyuki Okamoto, Toshio Sakai, Masao Inoue, Shuichi Yamasaki, Seiji Ishida, Satoshi Tsuneda, Akira Hirata