Abstract: Provided are modified influenza virus subtype 2 neuraminidase molecules lacking all or substantially all of the stalk region that form active, soluble tetrameric neuraminidase when expressed in host cells and vaccine compositions comprising the tetrameric neuraminidase or a nucleic acid encoding the modified monomeric influenza virus subtype 2 neuraminidase molecules that forms tetrameric NA when expressed in a cell. Also provided are methods of using the vaccine compositions to vaccinate or immunize a subject against influenza virus.

Abstract: The present disclosure relates to a method and a system for enabling real time location based personalized offer management to a customer. In one embodiment, the method identifies a plurality of customers likely visiting the store, and determines a plurality of relevant personalized bars that can be provided to the identified customers. Th.e method further receives real time information about the presence of customers within the store and provides the in-store customers with one or more real time recommendations of offers on products based on the usage of the relevant personalized offers. Thus, the method and system provides personalized promotional offer based on convenience of individual customers, customers interest on different products on real-time within the establishment. Further, the method and system also provides alternate offers to customers present within store and assess the promotional effectiveness of the campaign on a real-time basis.