Patents by Inventor Saurabh Nirantar
Saurabh Nirantar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240141427Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: September 27, 2023Publication date: May 2, 2024Applicant: ILLUMINA, INC.Inventors: Misha Golynskiy, Rahman Rahman Pour, Jiawen Li, Ryan Craig, Hamed Tabatabaei Ghomi, Saurabh Nirantar, Hsu Myat Noe, Lin Hui Chang, Yvonne Devadas, Jing Wen Lim, Kay Klausing, Humberto Rojo, Eric Murtfeldt, Chris Garcia
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Publication number: 20230383342Abstract: Embodiments of the present disclosure relate to kits, compositions, and methods for nucleic acid sequencing, for example, two-channel nucleic acid sequencing by synthesis using blue and green light excitation. In particular, unlabeled nucleotides for incorporation may be used in conjunction with affinity reagents containing detectable labels excitable by blue and/or green lights, for specific binding to each type of nucleotides incorporated.Type: ApplicationFiled: May 30, 2023Publication date: November 30, 2023Inventors: Xiaolin Wu, Patrick McCauley, Madushani Dharmarwardana, Saurabh Nirantar, Misha Golynskiy, Xiangyuan Yang, Ramesh Neelakandan, Benedict Mackworth, Carole Anastasi, Aathavan Karunakaran, Gery M. Vessere, David Bracher
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Publication number: 20230357731Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: March 13, 2023Publication date: November 9, 2023Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20230332197Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with an optionally substituted 3?vinyl blocking group. Also provided herein are methods to prepare such nucleotide and nucleoside molecules. Additionally, the present disclosure provides methods of using such blocked nucleosides and nucleotides in oligonucleotide synthesis and sequencing.Type: ApplicationFiled: March 29, 2023Publication date: October 19, 2023Inventors: Ramesh Neelakandan, Daniel Hartoyo Lukamto, Jean-Alexandre Richard, Yin Nah Teo, Xiangyuan Yang, Sadeer Salam, Jing Liang, Saurabh Nirantar
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Publication number: 20230212534Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: December 21, 2022Publication date: July 6, 2023Applicants: ILLUMINA, INC., ILLUMINA SINGAPORE PTE LTDInventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Patent number: 11634697Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: April 9, 2021Date of Patent: April 25, 2023Assignees: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20230047225Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.Type: ApplicationFiled: June 1, 2022Publication date: February 16, 2023Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Misha Golynskiy, Mariko Matsuura, Saurabh Nirantar, Hamed Tabatabaei Ghomi, Seth McDonald, Ryan Craig, Sergio Peisajovich, Kyrie Johnson, Kay Klausing, Eric Murtfeldt, Alexandra Exner
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Patent number: 11560552Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: August 9, 2021Date of Patent: January 24, 2023Assignees: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Publication number: 20220333146Abstract: Provided is a method including extending a ssDNA by sequentially adding a plurality of modified nucleoside triphosphates to the ssDNA, wherein the base of the modified nucleoside triphosphates includes a primary modification selected from (i) a primary polynucleotide attached to the base of the modified nucleoside triphosphate, and (ii) a site on the base for covalent attachment of a primary polynucleotide to the base, further comprising covalently attaching a primary polynucleotide to the base after the polymerizing.Type: ApplicationFiled: April 13, 2022Publication date: October 20, 2022Applicant: ILLUMINA SINGAPORE PTE. LTD.Inventors: Xiangyuan YANG, Saurabh NIRANTAR, Yin Nah TEO
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Publication number: 20220056424Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: August 9, 2021Publication date: February 24, 2022Applicants: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Publication number: 20210348141Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: April 9, 2021Publication date: November 11, 2021Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 11104888Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: October 31, 2019Date of Patent: August 31, 2021Assignees: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Publication number: 20210187469Abstract: Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from one or more scaffold DNA molecules and one or more scaffold polypeptides, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle.Type: ApplicationFiled: December 22, 2020Publication date: June 24, 2021Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Wayne N. GEORGE, Jonathan Mark BOUTELL, Mathieu LESSARD-VIGER, Saurabh NIRANTAR, Pietro GATTI LAFRANCONI, Seth MCDONALD, Yin Nah TEO, Maria Rogert BACIGALUPO, Sergio PEISAJOVICH, Eric BRUSTAD, Michael HOWARD, Xiangyuan YANG, M. Shane Bowen, Alex NEMIROSKI, Jeffrey BRODIN, Ludovic VINCENT, Lorenzo BERTI, Andrew A. BROWN, Fei SHEN, Lena STORMS, Olivia BENICE
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Patent number: 11001816Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: December 4, 2019Date of Patent: May 11, 2021Assignees: Illumina, Inc., Illumina Singapore PTE. LTD., Illumina Cambridge LimitedInventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20200181587Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: December 4, 2019Publication date: June 11, 2020Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabael Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20200131484Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: October 31, 2019Publication date: April 30, 2020Applicants: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Patent number: 8871686Abstract: The invention relates to methods of identifying a binding partner of a target molecule within a plurality of analyte molecules, including a plurality of peptides and/or proteins. The target molecule is physically combined with a target labeling nucleic acid molecule. Each member of the plurality of analyte molecules is physically linked to an analyte labeling nucleic acid molecule, each analyte labeling nucleic acid molecule comprising a selected nucleotide sequence. This specific nucleotide sequence may include a sequence encoding a peptide/protein combined therewith. The target molecule is contacted with the analyte molecules and a complex between the target molecule and an analyte molecule forms. The mixture is subdivided into compartments. The target labeling nucleic acid molecule and the analyte labeling nucleic acid molecule are linked and the plurality of compartments allowed to disintegrate. The linked nucleic acid molecule is retrieved and the sequence determined.Type: GrantFiled: July 7, 2010Date of Patent: October 28, 2014Assignee: Agency for Science, Technology and ResearchInventors: John Farid Ghadessy, Saurabh Nirantar
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Publication number: 20130143749Abstract: The present invention relates to methods of identifying a binding partner of a target molecule within a plurality of analyte molecules, including a plurality of peptides and/or proteins. The target molecule is physically combined with a target labeling nucleic acid molecule, which includes a specific nucleotide sequence. Where the target molecule is a peptide/protein this specific nucleotide sequence may include a sequence encoding the target molecule. Each member of the plurality of analyte molecules is physically linked to an analyte labeling nucleic acid molecule, each analyte labeling nucleic acid molecule comprising a selected nucleotide sequence. This specific nucleotide sequence may include a sequence encoding a peptide/protein combined therewith. The target molecule is contacted with the analyte molecules and a complex between the target molecule and an analyte molecule forms.Type: ApplicationFiled: July 7, 2010Publication date: June 6, 2013Inventors: John Farid Ghadessy, Saurabh Nirantar